eprintid: 1369788
rev_number: 31
eprint_status: archive
userid: 608
dir: disk0/01/36/97/88
datestamp: 2012-10-29 19:42:34
lastmod: 2021-09-19 22:26:26
status_changed: 2012-10-29 19:42:34
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: FitzHarris, G
title: Anaphase B precedes anaphase A in the mouse egg.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
keywords: Anaphase, Animals, Cell Division, Chromosome Segregation, Chromosomes, Kinesin, Kinetochores, Mice, Mitotic Spindle Apparatus, Oocytes, RNA, Messenger
note: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
abstract: Segregation of chromosomes at the time of cell division is achieved by the microtubules and associated molecules of the spindle. Chromosomes attach to kinetochore microtubules (kMTs), which extend from the spindle pole region to kinetochores assembled upon centromeric DNA. In most animal cells studied, chromosome segregation occurs as a result of kMT shortening, which causes chromosomes to move toward the spindle poles (anaphase A). Anaphase A is typically followed by a spindle elongation that further separates the chromosomes (anaphase B). The experiments presented here provide the first detailed analysis of anaphase in a live vertebrate oocyte and show that chromosome segregation is initially driven by a significant spindle elongation (anaphase B), which is followed by a shortening of kMTs to fully segregate the chromosomes (anaphase A). Loss of tension across kMTs at anaphase onset produces a force imbalance, allowing the bipolar motor kinesin-5 to drive early anaphase B spindle elongation and chromosome segregation. Early anaphase B spindle elongation determines the extent of chromosome segregation and the size of the resulting cells. The vertebrate egg therefore employs a novel mode of anaphase wherein spindle elongation caused by loss of k-fiber tension is harnessed to kick-start chromosome segregation prior to anaphase A.
date: 2012-03-06
official_url: http://dx.doi.org/10.1016/j.cub.2012.01.041
vfaculties: VFLS
oa_status: green
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article, Research Support, Non-U.S. Gov't
verified: verified_manual
elements_source: PubMed
elements_id: 393724
doi: 10.1016/j.cub.2012.01.041
pii: S0960-9822(12)00071-1
lyricists_name: Fitzharris, David
lyricists_id: DGFIT24
full_text_status: public
publication: Current Biology
volume: 22
number: 5
pagerange: 437 - 444
event_location: UK
issn: 0960-9822
citation:        FitzHarris, G;      (2012)    Anaphase B precedes anaphase A in the mouse egg.                   Current Biology , 22  (5)   437 - 444.    10.1016/j.cub.2012.01.041 <https://doi.org/10.1016/j.cub.2012.01.041>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1369788/1/1369788.pdf