eprintid: 1343868 rev_number: 37 eprint_status: archive userid: 608 dir: disk0/01/34/38/68 datestamp: 2012-03-22 19:37:18 lastmod: 2021-10-20 23:53:24 status_changed: 2016-03-10 14:40:48 type: article metadata_visibility: show item_issues_count: 0 creators_name: Voulgaris, I creators_name: O'Donnell, A creators_name: Harvey, LM creators_name: McNeil, B title: Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield? ispublished: pub divisions: UCL divisions: B04 divisions: C05 keywords: Adenosine Triphosphate, Biomass, Carbon Dioxide, Fungi, Glucose, NADH Dehydrogenase, Quaternary Ammonium Compounds, Superoxides note: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/. abstract: Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration. date: 2012-03-20 official_url: http://dx.doi.org/10.1038/srep00322 vfaculties: VENG oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_source: PubMed elements_id: 402741 doi: 10.1038/srep00322 lyricists_name: Voulgaris, Ioannis lyricists_id: IVOUL66 full_text_status: public publication: Scientific Reports volume: 2 article_number: 322 event_location: England issn: 2045-2322 citation: Voulgaris, I; O'Donnell, A; Harvey, LM; McNeil, B; (2012) Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield? Scientific Reports , 2 , Article 322. 10.1038/srep00322 <https://doi.org/10.1038/srep00322>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1343868/1/Voulgaris_Inactivating%20alternative%20NADH%20dehydrogenases%253A%20enhancing%20fungal%20bioprocesses%20by%20improving%20growth%20and%20biomass%20yield%253F.pdf