eprintid: 1343868
rev_number: 37
eprint_status: archive
userid: 608
dir: disk0/01/34/38/68
datestamp: 2012-03-22 19:37:18
lastmod: 2021-10-20 23:53:24
status_changed: 2016-03-10 14:40:48
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Voulgaris, I
creators_name: O'Donnell, A
creators_name: Harvey, LM
creators_name: McNeil, B
title: Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield?
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
keywords: Adenosine Triphosphate, Biomass, Carbon Dioxide, Fungi, Glucose, NADH Dehydrogenase, Quaternary Ammonium Compounds, Superoxides
note: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
abstract: Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration.
date: 2012-03-20
official_url: http://dx.doi.org/10.1038/srep00322
vfaculties: VENG
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_source: PubMed
elements_id: 402741
doi: 10.1038/srep00322
lyricists_name: Voulgaris, Ioannis
lyricists_id: IVOUL66
full_text_status: public
publication: Scientific Reports
volume: 2
article_number: 322
event_location: England
issn: 2045-2322
citation:        Voulgaris, I;    O'Donnell, A;    Harvey, LM;    McNeil, B;      (2012)    Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield?                   Scientific Reports , 2     , Article 322.  10.1038/srep00322 <https://doi.org/10.1038/srep00322>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1343868/1/Voulgaris_Inactivating%20alternative%20NADH%20dehydrogenases%253A%20enhancing%20fungal%20bioprocesses%20by%20improving%20growth%20and%20biomass%20yield%253F.pdf