%X Muscle-derived cells (MDCs) are a heterogeneous population consisting of cells that can
undergo myogenic differentiation; however, it has emerged that not all MDCs are restricted
to the myogenic lineage. This discovery may have many implications; for example, MDCs
may be a suitable alternative source of osteogenic cells for bone repair. The currently
accepted treatment for bone repair, bone grafting, is often associated with small amount of
obtainable bone. Much of the work published regarding the differential potential of MDCs
has not, to date, focused on the osteogenic pathway and even fewer studies have been
performed on human cells. In this thesis osteogenic MDCs were isolated by differential
adhesion to fibronectin (Fn) i.e. MDCsFn and compared with mesenchymal stem cells
(MSCs) in relation to their osteogenic potential. The osteogenic potential was assessed by
measuring mineralization and relevant gene- and protein- expression. MSCs and MDCsFn
had a similar pattern of ALP activity and expression. Furthermore, MSCs and MDCsFn
both showed mineralization after 3 weeks measured by Alizarin Red S. A qPCR Array
measuring the activity of 46 osteogenic genes also showed similarities in gene expression
between the two cell types; however, the MSCs showed a more consistent pattern between
patients, compared to MDCsFn. Titanium (Ti) has previously been used as a bone repair
scaffold in humans due to its osteoconductivity. The interaction between Ti, of various
roughness and hydrophilicity, and the two cell types, i.e. MSCs and MDCsFn, were
assessed with relation to biocompatibility. Interestingly, the hydrophilic, rough surface,
which has been described as superior in bone formation applications, showed higher levels
of cell death, both apoptosis and necrosis, compared to the other tested surfaces for both
cell types. In conclusion, due to the similarities between MDCsFn and MSCs there might
be possibilities to use the osteogenic fraction in future bone regenerative applications.
%A K.H. Carlqvist
%D 2011
%T The potential of muscle-derived progenitors on titanium scaffolds in bone regenerative applications
%I UCL (University College London)
%L discovery1301768