eprintid: 10206109 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/20/61/09 datestamp: 2025-03-14 13:34:49 lastmod: 2025-03-14 13:34:49 status_changed: 2025-03-14 13:34:49 type: article metadata_visibility: show sword_depositor: 699 creators_name: Petagine, Lucy creators_name: Zariwala, Mohammed G creators_name: Somavarapu, Satyanarayana creators_name: Chan, Stefanie Ho Yi creators_name: Kaya, Evrim A creators_name: Patel, Vinood B title: Oxidative stress in a cellular model of alcohol-related liver disease: protection using curcumin nanoformulations ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G08 keywords: Alcohol, Antioxidants, Curcumin, Liver, Mitochondria, Oxidative stress, Reactive oxygen species note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. abstract: Alcohol-related liver disease (ARLD) is a global health issue causing significant morbidity and mortality, due to lack of suitable therapeutic options. ARLD induces a spectrum of biochemical and cellular alterations, including chronic oxidative stress, mitochondrial dysfunction, and cell death, resulting in hepatic injury. Natural antioxidant compounds such as curcumin have generated interest in ARLD due to their ability to scavenge reactive oxygen species (ROS), however, therapy using these compounds is limited due to poor bioavailability and stability. Therefore, the aim of this study was to assess the antioxidant potential of free antioxidants and curcumin entrapped formulations against oxidative damage in an ARLD cell model. HepG2 (VL-17A) cells were treated with varying concentrations of alcohol (from 200 to 350 mM) and parameters of oxidative stress and mitochondrial function were assessed over 72 h. Data indicated 350 mM of ethanol led to a significant decrease in cell viability at 72 h, and a significant increase in ROS at 30 min. A substantial number of cells were in late apoptosis at 72 h, and a reduction in the mitochondrial membrane potential was also found. Pre-treatment with curcumin nanoformulations increased viability, as well as, reducing ROS at 2 h, 48 h and 72 h. In summary, antioxidants and entrapped nanoformulations of curcumin were able to ameliorate reduced cell viability and increased ROS caused by ethanol treatment. This demonstrates their potential at mitigating oxidative damage and warrants further investigation to evaluate their efficacy for ARLD therapy. date: 2025-03-05 date_type: published publisher: Springer Science and Business Media LLC official_url: https://doi.org/10.1038/s41598-025-91139-0 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2370498 doi: 10.1038/s41598-025-91139-0 lyricists_name: Somavarapu, Satyanarayana lyricists_id: SSOMA36 actors_name: Somavarapu, Satyanarayana actors_name: Harris, Jean actors_id: SSOMA36 actors_id: JAHAR68 actors_role: owner actors_role: impersonator full_text_status: public publication: Scientific Reports volume: 15 article_number: 7752 issn: 2045-2322 citation: Petagine, Lucy; Zariwala, Mohammed G; Somavarapu, Satyanarayana; Chan, Stefanie Ho Yi; Kaya, Evrim A; Patel, Vinood B; (2025) Oxidative stress in a cellular model of alcohol-related liver disease: protection using curcumin nanoformulations. Scientific Reports , 15 , Article 7752. 10.1038/s41598-025-91139-0 <https://doi.org/10.1038/s41598-025-91139-0>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10206109/1/s41598-025-91139-0.pdf