eprintid: 10206045 rev_number: 9 eprint_status: archive userid: 699 dir: disk0/10/20/60/45 datestamp: 2025-03-13 10:13:49 lastmod: 2025-03-13 10:13:49 status_changed: 2025-03-13 10:13:49 type: article metadata_visibility: show sword_depositor: 699 creators_name: Aynekin, Busra creators_name: Akbaş, Sinan creators_name: Gulec, Ayten creators_name: Gumus, Ummu Gulsum Ozgul creators_name: Guner, Abdullah Emre creators_name: Efthymiou, Stephanie creators_name: Houlden, Henry creators_name: Sayin, Gözde Yesil creators_name: Per, Huseyin title: Phenotypic variability in progressive encephalopathy with brain atrophy and thin corpus callosum: insights from two families ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: D07 divisions: F85 keywords: Microtubule; PEBAT; WES; Neurodevelopmental disorder note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: The cytoskeleton, composed of microtubules, intermediate filaments and actin filaments is vital for various cellular functions, particularly within the nervous system, where microtubules play a key role in intracellular transport, cell morphology, and synaptic plasticity. Tubulin-specific chaperones, including tubulin folding cofactors (TBCA, TBCB, TBCC, TBCD, TBCE), assist in the proper formation of α/β-tubulin heterodimers, essential for microtubule stability. Pathogenic variants in these chaperone-encoding genes, especially TBCD, have been linked to Progressive Encephalopathy with Brain Atrophy and Thin Corpus Callosum (PEBAT, OMIM #604,649), a severe neurodevelopmental disorder. We report three cases from two consanguineous families with varying clinical presentations of PEBAT syndrome due to homozygous pathogenic variants in the TBCD. In Family 1, two siblings (F1C1 and F1C2) harboring the homozygous c.2314C > T, p.(Arg772Cys) variant exhibited severe neurodevelopmental regression, spastic tetraplegia, seizures, and brain atrophy. In contrast, Family 2, Case 3 (F2C3), with the homozygous c.230A > G, p.(His77Arg) variant, presented a milder phenotype, including absence seizures, slight developmental delay, and less pronounced neuroanatomical abnormalities. These findings contribute to the expanding phenotypic spectrum of PEBAT and suggesting that modifier genes or epigenetic factors may influence disease severity. date: 2025-01-24 date_type: published publisher: Springer Verlag official_url: https://doi.org/10.1007/s10048-025-00799-7 full_text_type: other language: eng verified: verified_manual elements_id: 2358430 doi: 10.1007/s10048-025-00799-7 medium: Electronic pii: 10.1007/s10048-025-00799-7 lyricists_name: Houlden, Henry lyricists_name: Efthymiou, Stephanie lyricists_id: HJHOU44 lyricists_id: SEFTH99 actors_name: Efthymiou, Stephanie actors_id: SEFTH99 actors_role: owner funding_acknowledgements: [Wellcome Trust]; [MRC]; [MSA Trust]; [National Institute for Health Research University College London Hospitals Biomedical Research Centre NIHR-BRC]; [Michael J Fox Foundation (MJFF)]; [Rosetrees Trust]; [Dolby Family fund]; [Alzheimer's Research UK (ARUK)]; [MSA Coalition, Parkinson's disease society, Parkinson's Foundation]; [Fidelity Trust]; [Guarantors of Brain, Cerebral Palsy Alliance]; [Victoria Brain bank]; [NIH NeuroBioBank]; [Queen Square BrainBank,]; [MRC Brainbank Network]; [FARA]; [EAN] full_text_status: restricted publication: Neurogenetics volume: 26 article_number: 23 pages: 8 event_location: United States issn: 1364-6745 citation: Aynekin, Busra; Akbaş, Sinan; Gulec, Ayten; Gumus, Ummu Gulsum Ozgul; Guner, Abdullah Emre; Efthymiou, Stephanie; Houlden, Henry; ... Per, Huseyin; + view all <#> Aynekin, Busra; Akbaş, Sinan; Gulec, Ayten; Gumus, Ummu Gulsum Ozgul; Guner, Abdullah Emre; Efthymiou, Stephanie; Houlden, Henry; Sayin, Gözde Yesil; Per, Huseyin; - view fewer <#> (2025) Phenotypic variability in progressive encephalopathy with brain atrophy and thin corpus callosum: insights from two families. Neurogenetics , 26 , Article 23. 10.1007/s10048-025-00799-7 <https://doi.org/10.1007/s10048-025-00799-7>. document_url: https://discovery.ucl.ac.uk/id/eprint/10206045/1/Efthymiou_30%20December%20TBCD%20Manuscript.pdf