eprintid: 10205827 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/20/58/27 datestamp: 2025-03-11 12:27:38 lastmod: 2025-03-11 12:27:38 status_changed: 2025-03-11 12:27:38 type: article metadata_visibility: show sword_depositor: 699 creators_name: Abbondanzieri, EA creators_name: Badrinarayanan, AB creators_name: Barillà, D creators_name: Bell, SD creators_name: Blombach, F creators_name: Bouet, JY creators_name: Bulgheresi, S creators_name: Cao, QAD creators_name: Dame, RT creators_name: Dekker, C creators_name: Demuysere, M creators_name: Espéli, O creators_name: Fogg, PCM creators_name: Freddolino, PL creators_name: Ganji, M creators_name: Gerson, TM creators_name: Grainger, DC creators_name: Hamoen, LW creators_name: Harju, J creators_name: Hocher, A creators_name: Hustmyer, CM creators_name: Kaljevic, JK creators_name: Karney, MK creators_name: Kleckner, N creators_name: Laloux, G creators_name: Landick, R creators_name: Lioy, VS creators_name: Liu, WL creators_name: Liu, CL creators_name: Mäkelä, J creators_name: Meyer, AS creators_name: Noy, A creators_name: Pineau, MP creators_name: Premrajka, K creators_name: Racki, LR creators_name: Rashid, F-ZM creators_name: Schnetz, K creators_name: Schwab, S creators_name: Tišma, M creators_name: van der Sijs, AI creators_name: van Heesch, T creators_name: van Raaphorst, R creators_name: Vreede, J creators_name: Walker, AW creators_name: Walter, J-C creators_name: Weber, SC creators_name: Wiggins, PA creators_name: Wing, HJ creators_name: Xiao, J creators_name: Zhang, Z title: Future Directions of the Prokaryotic Chromosome Field ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: G03 keywords: Archaeal chromatin, archaeal chromosome, bacterial chromatin, bacterial chromosome, nucleoid note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes. date: 2025-02 date_type: published publisher: Blackwell Publishing official_url: https://doi.org/10.1111/mmi.15347 full_text_type: other language: eng verified: verified_manual elements_id: 2366253 doi: 10.1111/mmi.15347 medium: Print lyricists_name: Blombach, Fabian lyricists_id: FBLOM86 actors_name: Blombach, Fabian actors_id: FBLOM86 actors_role: owner funding_acknowledgements: [The Lorentz Center is funded by the Dutch Research Council (NWO) and Leiden University] full_text_status: restricted publication: Molecular Microbiology volume: 123 number: 2 pagerange: 89-100 event_location: England issn: 0950-382X citation: Abbondanzieri, EA; Badrinarayanan, AB; Barillà, D; Bell, SD; Blombach, F; Bouet, JY; Bulgheresi, S; ... Zhang, Z; + view all <#> Abbondanzieri, EA; Badrinarayanan, AB; Barillà, D; Bell, SD; Blombach, F; Bouet, JY; Bulgheresi, S; Cao, QAD; Dame, RT; Dekker, C; Demuysere, M; Espéli, O; Fogg, PCM; Freddolino, PL; Ganji, M; Gerson, TM; Grainger, DC; Hamoen, LW; Harju, J; Hocher, A; Hustmyer, CM; Kaljevic, JK; Karney, MK; Kleckner, N; Laloux, G; Landick, R; Lioy, VS; Liu, WL; Liu, CL; Mäkelä, J; Meyer, AS; Noy, A; Pineau, MP; Premrajka, K; Racki, LR; Rashid, F-ZM; Schnetz, K; Schwab, S; Tišma, M; van der Sijs, AI; van Heesch, T; van Raaphorst, R; Vreede, J; Walker, AW; Walter, J-C; Weber, SC; Wiggins, PA; Wing, HJ; Xiao, J; Zhang, Z; - view fewer <#> (2025) Future Directions of the Prokaryotic Chromosome Field. Molecular Microbiology , 123 (2) pp. 89-100. 10.1111/mmi.15347 <https://doi.org/10.1111/mmi.15347>. document_url: https://discovery.ucl.ac.uk/id/eprint/10205827/1/MMI-2024-19687.R1_Proof_hi.pdf