TY - JOUR TI - Contrastive learning of T cell receptor representations AV - public Y1 - 2025/01/15/ VL - 16 N1 - Copyright © 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). IS - 1 ID - discovery10205427 N2 - Computational prediction of the interaction of T cell receptors (TCRs) and their ligands is a grand challenge in immunology. Despite advances in high-throughput assays, specificity-labeled TCR data remain sparse. In other domains, the pre-training of language models on unlabeled data has been successfully used to address data bottlenecks. However, it is unclear how to best pre-train protein language models for TCR specificity prediction. Here, we introduce a TCR language model called SCEPTR (simple contrastive embedding of the primary sequence of T cell receptors), which is capable of data-efficient transfer learning. Through our model, we introduce a pre-training strategy combining autocontrastive learning and masked-language modeling, which enables SCEPTR to achieve its state-of-the-art performance. In contrast, existing protein language models and a variant of SCEPTR pre-trained without autocontrastive learning are outperformed by sequence alignment-based methods. We anticipate that contrastive learning will be a useful paradigm to decode the rules of TCR specificity. A record of this paper?s transparent peer review process is included in the supplemental information. SN - 2405-4712 UR - https://doi.org/10.1016/j.cels.2024.12.006 PB - Elsevier BV JF - Cell Systems KW - Protein language models; contrastive learning; TCR repertoire; T cell specificity; TCR; T cell receptor; representation learning A1 - Nagano, Yuta A1 - Pyo, Andrew GT A1 - Milighetti, Martina A1 - Henderson, James A1 - Shawe-Taylor, John A1 - Chain, Benny A1 - Tiffeau-Mayer, Andreas ER -