eprintid: 10204992 rev_number: 9 eprint_status: archive userid: 699 dir: disk0/10/20/49/92 datestamp: 2025-02-19 08:34:56 lastmod: 2025-02-19 08:34:56 status_changed: 2025-02-19 08:34:56 type: article metadata_visibility: show sword_depositor: 699 creators_name: Si, Ruxue creators_name: Hu, Tingting creators_name: Williams, Gareth R creators_name: Yang, Yu creators_name: Yang, Shuqing creators_name: Yan, Dan creators_name: Liang, Ruizheng creators_name: Ji, Weiping title: Coupling Probiotics with CaO2 Nanoparticle-Loaded CoFeCe-LDH Nanosheets to Remodel the Tumor Microenvironment for Precise Chemodynamic Therapy ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G08 keywords: chemodynamic therapy, layered double hydroxides, probiotics, tumor microenvironment‐responsive, tumor‐targeting performance note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Chemodynamic therapy (CDT) has become an emerging cancer treatment strategy with advantages of tumor-specificity, high selectivity, and low systemic toxicity. However, it usually suffers from low therapeutic efficacy. This is caused by low hydroxyl radical (·OH) yield arising because of the relatively high pH, overexpressed glutathione, and low H2O2 concentration in the tumor microenvironment (TME). Herein, a probiotic metabolism-initiated pH reduction and H2O2 supply-enhanced CDT strategy is reported to eradicate tumors by generating ·OH, in which Lactobacillus acidophilus is coupled with CoFeCe-layered double hydroxide nanosheets loaded with CaO2 nanoparticles (NPs) as a chemodynamic platform for high-efficiency CDT (CaO2/LDH@L. acidophilus). Owing to the hypoxia tropism of L. acidophilus, CaO2/LDH@L. acidophilus exhibits increased accumulation at tumor sites compared with the CaO2/LDH. The CaO2 NPs loaded on CoFeCe-LDH nanosheets are decomposed into H2O2 in the TME. L. acidophilus metabolite-induced pH reduction (<5.5) and CaO2-mediated in situ H2O2 generation synergistically boost ·OH generation activity of the CoFeCe-LDH nanosheets, effectively damaging cancer cells and ablating tumors with a tumor inhibition rate of 96.4%, 2.32-fold higher than that of CaO2/LDH. This work demonstrates that probiotics can function as a tumor-targeting platform to remodel the TME and amplify ROS generation for highly efficient and precise CDT. date: 2025-01-27 date_type: published publisher: Wiley-Blackwell official_url: https://doi.org/10.1002/adhm.202403373 full_text_type: other language: eng verified: verified_manual elements_id: 2341871 doi: 10.1002/adhm.202403373 medium: Print-Electronic lyricists_name: Williams, Gareth lyricists_id: GWILL02 actors_name: Williams, Gareth actors_id: GWILL02 actors_role: owner funding_acknowledgements: 22288102 [National Natural Science Foundation of China]; 52322317 [Excellent Young Scientists Fund]; XK1803-05 [Fundamental Research Funds for the Central Universities of Beijing University of Chemical Technology]; LTGY23H030003 [Natural Science Foundation of Zhejiang Province]; zyyzdxk-2023003 [Key Discipline of the National Administration of Traditional Chinese Medicine]; 2022-051 [Youth Beijing Scholar] full_text_status: restricted publication: Advanced Healthcare Materials volume: 14 number: 3 article_number: e2403373 event_location: Germany issn: 2192-2640 citation: Si, Ruxue; Hu, Tingting; Williams, Gareth R; Yang, Yu; Yang, Shuqing; Yan, Dan; Liang, Ruizheng; Si, Ruxue; Hu, Tingting; Williams, Gareth R; Yang, Yu; Yang, Shuqing; Yan, Dan; Liang, Ruizheng; Ji, Weiping; - view fewer <#> (2025) Coupling Probiotics with CaO2 Nanoparticle-Loaded CoFeCe-LDH Nanosheets to Remodel the Tumor Microenvironment for Precise Chemodynamic Therapy. Advanced Healthcare Materials , 14 (3) , Article e2403373. 10.1002/adhm.202403373 <https://doi.org/10.1002/adhm.202403373>. document_url: https://discovery.ucl.ac.uk/id/eprint/10204992/1/Williams_Manuscript_Revised-highlight.pdf