eprintid: 10202807
rev_number: 6
eprint_status: archive
userid: 699
dir: disk0/10/20/28/07
datestamp: 2025-01-03 10:26:35
lastmod: 2025-01-03 10:26:35
status_changed: 2025-01-03 10:26:35
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Picco, Francesca
creators_name: Zeboudj, Lynda
creators_name: Oggero, Silvia
creators_name: Prato, Vincenzo
creators_name: Burgoyne, Thomas
creators_name: Gamper, Nikita
creators_name: Malcangio, Marzia
title: Macrophage to neuron communication via extracellular vesicles in neuropathic pain conditions
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D08
keywords: Neuropathic pain, DRG neurons, Macrophages, Extracellular vesicles, microRNAs, Cytokines
note: © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
abstract: Neuropathic pain following peripheral nerve injury results from maladaptive changes in neurons and immune cells contribution to mechanisms underlying chronic pain. Specifically, in dorsal root ganglia (DRG), sensory neuron cell bodies release extracellular vesicles (EVs) which promote pro-inflammatory macrophage accumulation that facilitates nociceptive signalling. Here, we show that macrophages shuttle EVs to neurons. Indeed, bone marrow-derived macrophages (BMDMs) release EVs containing microRNA-155 (miR-155) which are taken up by cultured sensory neurons. EV-mediated transfer of miR-155 suppresses phosphatase Ship1 expression and increases cytokine interleukin-6 (IL-6) contents. Intrathecal-injected BMDM-derived EVs accumulate in lumbar DRG and EVs containing miR-155 antagomir result in Ship1 upregulation, Il6 downregulation in neurons in concomitance to attenuation of neuropathic mechanical hypersensitivity. These data suggest that, under neuropathic conditions, pro-inflammatory macrophages shuttle EV-containing miR-155 to neurons and contribute to the expression of pronociceptive IL-6 in DRG.
date: 2025-01-15
date_type: published
publisher: Elsevier BV
official_url: https://doi.org/10.1016/j.heliyon.2024.e41268
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2344493
doi: 10.1016/j.heliyon.2024.e41268
lyricists_name: Burgoyne, Thomas
lyricists_id: TBURG58
actors_name: Burgoyne, Thomas
actors_id: TBURG58
actors_role: owner
full_text_status: public
publication: Heliyon
volume: 11
number: 1
article_number: e41268
issn: 2405-8440
citation:        Picco, Francesca;    Zeboudj, Lynda;    Oggero, Silvia;    Prato, Vincenzo;    Burgoyne, Thomas;    Gamper, Nikita;    Malcangio, Marzia;      (2025)    Macrophage to neuron communication via extracellular vesicles in neuropathic pain conditions.                   Heliyon , 11  (1)    , Article e41268.  10.1016/j.heliyon.2024.e41268 <https://doi.org/10.1016/j.heliyon.2024.e41268>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10202807/1/Macrophage%20to%20neuron%20communication.pdf