TY - INPR SN - 0305-1048 Y1 - 2024/11/20/ TI - InterPro: the protein sequence classification resource in 2025 AV - public N2 - InterPro (https://www.ebi.ac.uk/interpro) is a freely accessible resource for the classification of protein sequences into families. It integrates predictive models, known as signatures, from multiple member databases to classify sequences into families and predict the presence of domains and significant sites. The InterPro database provides annotations for over 200 million sequences, ensuring extensive coverage of UniProtKB, the standard repository of protein sequences, and includes mappings to several other major resources, such as Gene Ontology (GO), Protein Data Bank in Europe (PDBe) and the AlphaFold Protein Structure Database. In this publication, we report on the status of InterPro (version 101.0), detailing new developments in the database, associated web interface and software. Notable updates include the increased integration of structures predicted by AlphaFold and the enhanced description of protein families using artificial intelligence. Over the past two years, more than 5000 new InterPro entries have been created. The InterPro website now offers access to 85 000 protein families and domains from its member databases and serves as a long-term archive for retired databases. InterPro data, software and tools are freely available. UR - https://doi.org/10.1093/nar/gkae1082 ID - discovery10200737 N1 - © The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. A1 - Blum, Matthias A1 - Andreeva, Antonina A1 - Florentino, Laise Cavalcanti A1 - Chuguransky, Sara Rocio A1 - Grego, Tiago A1 - Hobbs, Emma A1 - Pinto, Beatriz Lazaro A1 - Orr, Ailsa A1 - Paysan-Lafosse, Typhaine A1 - Ponamareva, Irina A1 - Salazar, Gustavo A A1 - Bordin, Nicola A1 - Bork, Peer A1 - Bridge, Alan A1 - Colwell, Lucy A1 - Gough, Julian A1 - Haft, Daniel H A1 - Letunic, Ivica A1 - Llinares-López, Felipe A1 - Marchler-Bauer, Aron A1 - Meng-Papaxanthos, Laetitia A1 - Mi, Huaiyu A1 - Natale, Darren A A1 - Orengo, Christine A A1 - Pandurangan, Arun P A1 - Piovesan, Damiano A1 - Rivoire, Catherine A1 - Sigrist, Christian JA A1 - Thanki, Narmada A1 - Thibaud-Nissen, Françoise A1 - Thomas, Paul D A1 - Tosatto, Silvio CE A1 - Wu, Cathy H A1 - Bateman, Alex PB - Oxford University Press (OUP) JF - Nucleic Acids Research ER -