eprintid: 10200127
rev_number: 9
eprint_status: archive
userid: 699
dir: disk0/10/20/01/27
datestamp: 2024-11-15 10:49:53
lastmod: 2024-11-15 10:49:53
status_changed: 2024-11-15 10:49:53
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Han, Kunyuan
creators_name: Cheng, Yunzhang
creators_name: Han, Qinglin
creators_name: Chen, Jishizhan
title: Extraction of type I collagen and development of collagen methacryloyl (ColMA)/PEGDA ink for digital light processing printing
ispublished: pub
divisions: UCL
divisions: B04
divisions: F45
keywords: Ink, Digital light processing, Additive manufacturing, Collagen, Hydrogel, Biomedical engineering
note: © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
abstract: The fabrication of three-dimensional (3D) biostructures through additive manufacturing relies on the critical role of ink development. With the growing demand for high-resolution manufacturing, digital light processing (DLP) technology has emerged as a promising technique requiring specialised photosensitive inks. Although gelatine methacryloyl (GelMA) has been the primary option for DLP, its mechanical properties, biocompatibility, and low stability still present limitations. The development of collagen-based ink is thus in high demand for a wider stiffness adjustment range, native bioactivities, and versatility in biomedical engineering applications. In this paper, we report a rapid and low-cost protocol for collagen methacryloyl (ColMA)/poly(ethylene glycol) diacrylate (PEGDA) ink for DLP printing. The ink demonstrated the highest printing resolution of ∼50 μm by using 405 nm visible light. The printability, mechanical properties and cell viability of the DLP-printed ColMA/PEGDA structures were comprehensively evaluated. The printed ColMA/PEGDA structures reached a compressive modulus over 100 kPa with 0.6 wt% collagen. The printed ColMA/PEGDA scaffolds promoted the attachment and proliferation of 3 T3 fibroblasts, demonstrating their potential in future applications in biomedical engineering.
date: 2024-12
date_type: published
publisher: Elsevier BV
official_url: http://dx.doi.org/10.1016/j.ijbiomac.2024.137253
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2335425
doi: 10.1016/j.ijbiomac.2024.137253
lyricists_name: Chen, Jishizhan
lyricists_id: JCHEI07
actors_name: Chen, Jishizhan
actors_id: JCHEI07
actors_role: owner
full_text_status: public
publication: International Journal of Biological Macromolecules
volume: 282
number: 6
article_number: 137253
issn: 0141-8130
citation:        Han, Kunyuan;    Cheng, Yunzhang;    Han, Qinglin;    Chen, Jishizhan;      (2024)    Extraction of type I collagen and development of collagen methacryloyl (ColMA)/PEGDA ink for digital light processing printing.                   International Journal of Biological Macromolecules , 282  (6)    , Article 137253.  10.1016/j.ijbiomac.2024.137253 <https://doi.org/10.1016/j.ijbiomac.2024.137253>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10200127/7/Chen_1-s2.0-S0141813024080620-main.pdf