eprintid: 10199769
rev_number: 7
eprint_status: archive
userid: 699
dir: disk0/10/19/97/69
datestamp: 2024-11-08 09:20:59
lastmod: 2024-11-08 09:20:59
status_changed: 2024-11-08 09:20:59
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Bangash, M Ali
creators_name: Cubuk, Cankut
creators_name: Iseppon, Federico
creators_name: Haroun, Rayan
creators_name: Garcia, Chloe
creators_name: Luiz, Ana P
creators_name: Arcangeletti, Manuel
creators_name: Gossage, Samuel J
creators_name: Santana-Varela, Sonia
creators_name: Cox, James J
creators_name: Lewis, Myles J
creators_name: Wood, John N
creators_name: Zhao, Jing
title: Analgesic targets identified in mouse sensory neuron somata and terminal pain translatomes
ispublished: pub
divisions: UCL
divisions: B02
divisions: C10
divisions: D17
divisions: G95
note: © 2024 The Authors. Published by Elsevier Inc. under a Creative Commons license (http://creativecommons.org/licenses/by/4.0/).
abstract: The relationship between transcription and protein expression is complex. We identified polysome-associated RNA transcripts in the somata and central terminals of mouse sensory neurons in control, painful (plus nerve growth factor), and pain-free conditions (Nav1.7-null mice). The majority (98%) of translated transcripts are shared between male and female mice in both the somata and terminals. Some transcripts are highly enriched in the somata or terminals. Changes in the translatome in painful and pain-free conditions include novel and known regulators of pain pathways. Antisense knockdown of selected somatic and terminal polysome-associated transcripts that correlate with pain states diminished pain behavior. Terminal-enriched transcripts included those encoding synaptic proteins (e.g., synaptotagmin), non-coding RNAs, transcription factors (e.g., Znf431), proteins associated with transsynaptic trafficking (HoxC9), GABA-generating enzymes (Gad1 and Gad2), and neuropeptides (Penk). Thus, central terminal translation may well be a significant regulatory locus for peripheral input from sensory neurons.
date: 2024-08-27
date_type: published
publisher: CELL PRESS
official_url: http://dx.doi.org/10.1016/j.celrep.2024.114614
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2306672
doi: 10.1016/j.celrep.2024.114614
medium: Print-Electronic
pii: S2211-1247(24)00964-1
lyricists_name: Zhao, Jing
lyricists_name: Wood, John
lyricists_name: Gossage, Samuel
lyricists_name: Cox, James
lyricists_id: JZHAO52
lyricists_id: JNWOO78
lyricists_id: SJGOS79
lyricists_id: JCOXX25
actors_name: Zhao, Jing
actors_id: JZHAO52
actors_role: owner
funding_acknowledgements: 21950 [Versus Arthritis UK]; MR/V012509/1 [Medical Research Council]; 571476 [Medical Research Council]; 185341 [Cancer Research UK]; NIHR 203330 [National Institute for Health Research Barts Biomedical Research Centre]
full_text_status: public
publication: Cell Reports
volume: 43
number: 8
article_number: 114614
pages: 21
event_location: United States
citation:        Bangash, M Ali;    Cubuk, Cankut;    Iseppon, Federico;    Haroun, Rayan;    Garcia, Chloe;    Luiz, Ana P;    Arcangeletti, Manuel;                         ... Zhao, Jing; + view all <#>        Bangash, M Ali;  Cubuk, Cankut;  Iseppon, Federico;  Haroun, Rayan;  Garcia, Chloe;  Luiz, Ana P;  Arcangeletti, Manuel;  Gossage, Samuel J;  Santana-Varela, Sonia;  Cox, James J;  Lewis, Myles J;  Wood, John N;  Zhao, Jing;   - view fewer <#>    (2024)    Analgesic targets identified in mouse sensory neuron somata and terminal pain translatomes.                   Cell Reports , 43  (8)    , Article 114614.  10.1016/j.celrep.2024.114614 <https://doi.org/10.1016/j.celrep.2024.114614>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10199769/1/2024%20Ali%20Cell%20Reports.pdf