TY  - JOUR
N2  - The relationship between transcription and protein expression is complex. We identified polysome-associated RNA transcripts in the somata and central terminals of mouse sensory neurons in control, painful (plus nerve growth factor), and pain-free conditions (Nav1.7-null mice). The majority (98%) of translated transcripts are shared between male and female mice in both the somata and terminals. Some transcripts are highly enriched in the somata or terminals. Changes in the translatome in painful and pain-free conditions include novel and known regulators of pain pathways. Antisense knockdown of selected somatic and terminal polysome-associated transcripts that correlate with pain states diminished pain behavior. Terminal-enriched transcripts included those encoding synaptic proteins (e.g., synaptotagmin), non-coding RNAs, transcription factors (e.g., Znf431), proteins associated with transsynaptic trafficking (HoxC9), GABA-generating enzymes (Gad1 and Gad2), and neuropeptides (Penk). Thus, central terminal translation may well be a significant regulatory locus for peripheral input from sensory neurons.
ID  - discovery10199769
UR  - http://dx.doi.org/10.1016/j.celrep.2024.114614
PB  - CELL PRESS
JF  - Cell Reports
A1  - Bangash, M Ali
A1  - Cubuk, Cankut
A1  - Iseppon, Federico
A1  - Haroun, Rayan
A1  - Garcia, Chloe
A1  - Luiz, Ana P
A1  - Arcangeletti, Manuel
A1  - Gossage, Samuel J
A1  - Santana-Varela, Sonia
A1  - Cox, James J
A1  - Lewis, Myles J
A1  - Wood, John N
A1  - Zhao, Jing
TI  - Analgesic targets identified in mouse sensory neuron somata and terminal pain translatomes
AV  - public
Y1  - 2024/08/27/
VL  - 43
EP  - 21
IS  - 8
N1  - © 2024 The Authors. Published by Elsevier Inc. under a Creative Commons license (http://creativecommons.org/licenses/by/4.0/).
ER  -