TY  - JOUR
JF  - Translational Oncology
KW  - EGFR
KW  -  Liquid biopsy
KW  -  Lung cancer
KW  -  Pleural effusion
A1  - Vukovic, Miodrag
A1  - Tanic, Miljana
A1  - Damjanovic, Ana
A1  - Pavlovic, Marijana
A1  - Stanojevic, Aleksandra
A1  - Zivic, Katarina
A1  - Karadzic, Valentina
A1  - Jankovic, Radmila
A1  - Cavic, Milena
ID  - discovery10198733
N2  - Background:
The use tumor-derived cell-free DNA extracted from body fluids is being evaluated for genetic testing in lung cancer. The aim of this study was to explore the feasibility and utility of implementation of EGFR molecular testing from pleural effusions in non-small cell lung cancer in the clinical diagnostics workflow.//
Patients and methods:
This study included patients diagnosed with primary lung adenocarcinoma in the period July 2016 to June 2023. EGFR mutation testing was performed by qPCR (Cobas®) and dPCR. Testing was performed from 211 plasma samples when tissue was unavailable at diagnosis, and from 301 plasma samples and 18 pleural effusions at progression on first/second generation of EGFR TKIs. Descriptive methods of statistical analysis were used to summarize the sample data. Fisher's exact test, McNemar's test, Cohen's kappa tests were used for statistical analyses. Two-sided p-values <0.05 were considered statistically significant.//
Results:
A significantly higher detection rate of the T790M mutation in pleural effusion was obtained compared to blood (50% and 20%, p=0.047). When comparing the detection success rate of the resistant T790M mutation in pleural effusion and blood, a statistically significant difference was obtained in favor of pleural effusion (50% vs. 21.87%, p=0.01).//
Conclusions:
Superior performance of pleural effusions compared to blood plasma was shown both in the analysis of success rate and in the detection of the resistant T790M mutation, at progression on EGFR TKIs. Pleural effusion should be considered in this setting whenever available, especially in countries with limited health resources.
SN  - 1936-5233
UR  - https://doi.org/10.1016/j.tranon.2023.101772
PB  - ELSEVIER SCIENCE INC
N1  - © The Author(s), 2023. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CC-BY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
TI  - EGFR mutation testing from pleural effusions of non-small cell lung cancer patients at the institute for oncology and radiology of Serbia
EP  - 8
AV  - public
Y1  - 2023/11//
VL  - 37
ER  -