eprintid: 10198429 rev_number: 6 eprint_status: archive userid: 699 dir: disk0/10/19/84/29 datestamp: 2024-10-15 14:03:55 lastmod: 2024-10-15 14:03:55 status_changed: 2024-10-15 14:03:55 type: article metadata_visibility: show sword_depositor: 699 creators_name: In ‘t Zandt, R creators_name: Persson, IM creators_name: Tibiletti, M creators_name: von Wachenfeldt, K creators_name: Parker, GJM creators_name: Olsson, LE title: Contrast enhanced longitudinal changes observed in an experimental bleomycin-induced lung fibrosis rat model by radial DCE-MRI at 9.4T ispublished: pub divisions: UCL divisions: B04 divisions: F42 keywords: Animals, Bleomycin, Male, Magnetic Resonance Imaging, Pulmonary Fibrosis, Rats, Sprague-Dawley, Rats, Contrast Media, Disease Models, Animal, Lung note: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ abstract: Identifying biomarkers in fibrotic lung disease is key for early anti-fibrotic intervention. Dynamic contrast-enhanced (DCE) MRI offers valuable perfusion-related insights in fibrosis but adapting human MRI methods to rodents poses challenges. Here, we explored these translational challenges for the inflammatory and fibrotic phase of a bleomycin lung injury model in rats. Eleven male Sprague-Dawley rats received a single intratracheal dose of bleomycin (1000iU), four control rats received saline. Imaging was performed on days 7 and 28 post-induction. Ultra-short echo time imaging was used to image the lung for 7 minutes after which Clariscan was injected intravenously. Lung signal changes were measured for an additional 21 minutes. Images were reconstructed with a sliding-window approach, providing a temporal resolution of 10 seconds per image. After imaging on day 28, animals were euthanized, and lungs were collected for histology. Bleomycin-exposed rats initially exhibited reduced body weight, recovering to control levels after 20 days. Lung volume increased in bleomycin animals from 4.4±0.9 ml in controls to 5.5±0.5 ml and 6.5±1.2 ml on day 7 and 28. DCE-MRI showed no change of initial gradient of relative enhancement in the curves between controls and bleomycin animals on day 7 and 28 post-induction. On day 7, the DCE-MRI washout phase in bleomycin animals had higher signals than the saline group and than observed at a later time point. Lung pixels were binned in 7 enhancement classes. On day 28, the size of low relative enhancement bins almost doubled in volume compared to controls and animals on day 7 post-induction. Histology on day 28 suggests that findings could be explained by changes in lung tissue density due to lung volume increase. Adapting this clinical MRI method to rodents at 9.4T remains a challenge. Future studies may benefit from lower field strength MRI combined with higher temporal resolution DCE-MRI. date: 2024-09-01 date_type: published publisher: Public Library of Science (PLoS) official_url: http://dx.doi.org/10.1371/journal.pone.0310643 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2327099 doi: 10.1371/journal.pone.0310643 medium: Electronic-eCollection pii: PONE-D-23-43281 lyricists_name: Parker, Geoffrey lyricists_id: GPARK63 actors_name: Parker, Geoffrey actors_id: GPARK63 actors_role: owner funding_acknowledgements: 116106 [Innovative Health Initiative] full_text_status: public publication: PLOS One volume: 19 article_number: e0310643 event_location: United States editors_name: Wu, Minghua citation: In ‘t Zandt, R; Persson, IM; Tibiletti, M; von Wachenfeldt, K; Parker, GJM; Olsson, LE; (2024) Contrast enhanced longitudinal changes observed in an experimental bleomycin-induced lung fibrosis rat model by radial DCE-MRI at 9.4T. PLOS One , 19 , Article e0310643. 10.1371/journal.pone.0310643 <https://doi.org/10.1371/journal.pone.0310643>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10198429/1/Rene%20paper.pdf