eprintid: 10198355
rev_number: 7
eprint_status: archive
userid: 699
dir: disk0/10/19/83/55
datestamp: 2024-10-11 09:35:09
lastmod: 2024-10-11 09:35:09
status_changed: 2024-10-11 09:35:09
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Ovari, Gyorgy
creators_name: Johnson, Thomas F
creators_name: Foroutan, Farzad
creators_name: Malmquist, Gunnar
creators_name: Townsend, Matthew
creators_name: Bracewell, Daniel G
title: Fabrication of electrospun ion exchanger adsorbents with morphologies designed for the separation of proteins and plasmid DNA
ispublished: pub
divisions: UCL
divisions: B04
divisions: F47
keywords: Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Chemistry, Analytical, Biochemistry & Molecular Biology, Chemistry, Electrospun adsorbents, Electrospinning, Protein adsorption, Plasmid DNA adsorption, X-ray computed tomography, BET surface area, MEMBRANE CHROMATOGRAPHY, NANOFIBER ADSORBENTS, PURIFICATION, VECTOR
note: © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
abstract: Electrospun cellulose adsorbents are an emergent class of materials applied to a variety of bioprocess separations as an analogue to conventional packed bed chromatography. Electrospun adsorbents have proven to be effective as rapid cycling media, enabling high throughput separation of proteins and viral vectors without compromising selectivity and recovery. However, there is a current lack of knowledge in relation to the manipulation and control of electrospun adsorbent structure with function and performance to cater to the separation needs of emerging, diverse biological products. In this study, a series of electrospun cellulose adsorbents were fabricated by adjusting their manufacturing conditions. A range of fiber diameters (400 to 600 nm) was created by changing the electrospinning polymer solution. Additionally, a range of porosities (0.4 to 0.7 v/v) was achieved by varying the laminating pressures on the electrospun sheets. The adsorbents were functionalized with different degrees of quaternary amine ligand density to create 18 prototype anion exchangers. Their morphology was characterized by BET nitrogen adsorption surface area, X-ray computed tomography, capillary flow porometry and scanning electron microscopy measurements. The physical characteristics of the adsorbents were used in an adapted semi-empirical model and compared to measured permeability data. Permeabilities of prototypes ranged from 10−2 to 10−4 mDarcy. The measured data showed good adherence to modelled data with possible improvements in acquiring wet adsorbent characteristics instead of dried material. Finally, the electrospun adsorbents were characterized for their binding capacity of model proteins of different sizes (diameters of 3.5 nm and 8.9 nm) and plasmid DNA. Static binding capacities ranged from 5 mg/ml to 25 mg/ml for the proteins and plasmid DNA and showed <20 % deviation from monolayer coverage based on BET surface area. Therefore, it was concluded that the electrospun adsorbents most likely adsorb monolayers of proteins and plasmid DNA on the surface with minimal steric hindrance.
date: 2024-10-11
date_type: published
publisher: Elsevier BV
official_url: http://dx.doi.org/10.1016/j.chroma.2024.465268
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2307407
doi: 10.1016/j.chroma.2024.465268
medium: Print-Electronic
pii: S0021-9673(24)00642-3
lyricists_name: Bracewell, Daniel
lyricists_name: Johnson, Thomas
lyricists_name: Ovari, Gyorgy
lyricists_id: DGBRA75
lyricists_id: TJOHN15
lyricists_id: GOVAR65
actors_name: Johnson, Thomas
actors_id: TJOHN15
actors_role: owner
funding_acknowledgements: EP/S021868/1 [EPSRC Centre for Doctoral Training in Bioprocess Engineering Leadership (Complex Biological Products Manufacture)]; [Cytiva]; MR/W004399/1 [Medical Research Council (MRC) Innovation Scholarship Grant]; EP/T02593X/1 [EPSRC]; [National X-ray Computed Tomography (NXCT)]
full_text_status: public
publication: Journal of Chromatography A
volume: 1734
article_number: 465268
event_location: Netherlands
issn: 0021-9673
citation:        Ovari, Gyorgy;    Johnson, Thomas F;    Foroutan, Farzad;    Malmquist, Gunnar;    Townsend, Matthew;    Bracewell, Daniel G;      (2024)    Fabrication of electrospun ion exchanger adsorbents with morphologies designed for the separation of proteins and plasmid DNA.                   Journal of Chromatography A , 1734     , Article 465268.  10.1016/j.chroma.2024.465268 <https://doi.org/10.1016/j.chroma.2024.465268>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10198355/1/1-s2.0-S0021967324006423-main.pdf