eprintid: 10198355 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/19/83/55 datestamp: 2024-10-11 09:35:09 lastmod: 2024-10-11 09:35:09 status_changed: 2024-10-11 09:35:09 type: article metadata_visibility: show sword_depositor: 699 creators_name: Ovari, Gyorgy creators_name: Johnson, Thomas F creators_name: Foroutan, Farzad creators_name: Malmquist, Gunnar creators_name: Townsend, Matthew creators_name: Bracewell, Daniel G title: Fabrication of electrospun ion exchanger adsorbents with morphologies designed for the separation of proteins and plasmid DNA ispublished: pub divisions: UCL divisions: B04 divisions: F47 keywords: Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Chemistry, Analytical, Biochemistry & Molecular Biology, Chemistry, Electrospun adsorbents, Electrospinning, Protein adsorption, Plasmid DNA adsorption, X-ray computed tomography, BET surface area, MEMBRANE CHROMATOGRAPHY, NANOFIBER ADSORBENTS, PURIFICATION, VECTOR note: © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). abstract: Electrospun cellulose adsorbents are an emergent class of materials applied to a variety of bioprocess separations as an analogue to conventional packed bed chromatography. Electrospun adsorbents have proven to be effective as rapid cycling media, enabling high throughput separation of proteins and viral vectors without compromising selectivity and recovery. However, there is a current lack of knowledge in relation to the manipulation and control of electrospun adsorbent structure with function and performance to cater to the separation needs of emerging, diverse biological products. In this study, a series of electrospun cellulose adsorbents were fabricated by adjusting their manufacturing conditions. A range of fiber diameters (400 to 600 nm) was created by changing the electrospinning polymer solution. Additionally, a range of porosities (0.4 to 0.7 v/v) was achieved by varying the laminating pressures on the electrospun sheets. The adsorbents were functionalized with different degrees of quaternary amine ligand density to create 18 prototype anion exchangers. Their morphology was characterized by BET nitrogen adsorption surface area, X-ray computed tomography, capillary flow porometry and scanning electron microscopy measurements. The physical characteristics of the adsorbents were used in an adapted semi-empirical model and compared to measured permeability data. Permeabilities of prototypes ranged from 10−2 to 10−4 mDarcy. The measured data showed good adherence to modelled data with possible improvements in acquiring wet adsorbent characteristics instead of dried material. Finally, the electrospun adsorbents were characterized for their binding capacity of model proteins of different sizes (diameters of 3.5 nm and 8.9 nm) and plasmid DNA. Static binding capacities ranged from 5 mg/ml to 25 mg/ml for the proteins and plasmid DNA and showed <20 % deviation from monolayer coverage based on BET surface area. Therefore, it was concluded that the electrospun adsorbents most likely adsorb monolayers of proteins and plasmid DNA on the surface with minimal steric hindrance. date: 2024-10-11 date_type: published publisher: Elsevier BV official_url: http://dx.doi.org/10.1016/j.chroma.2024.465268 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2307407 doi: 10.1016/j.chroma.2024.465268 medium: Print-Electronic pii: S0021-9673(24)00642-3 lyricists_name: Bracewell, Daniel lyricists_name: Johnson, Thomas lyricists_name: Ovari, Gyorgy lyricists_id: DGBRA75 lyricists_id: TJOHN15 lyricists_id: GOVAR65 actors_name: Johnson, Thomas actors_id: TJOHN15 actors_role: owner funding_acknowledgements: EP/S021868/1 [EPSRC Centre for Doctoral Training in Bioprocess Engineering Leadership (Complex Biological Products Manufacture)]; [Cytiva]; MR/W004399/1 [Medical Research Council (MRC) Innovation Scholarship Grant]; EP/T02593X/1 [EPSRC]; [National X-ray Computed Tomography (NXCT)] full_text_status: public publication: Journal of Chromatography A volume: 1734 article_number: 465268 event_location: Netherlands issn: 0021-9673 citation: Ovari, Gyorgy; Johnson, Thomas F; Foroutan, Farzad; Malmquist, Gunnar; Townsend, Matthew; Bracewell, Daniel G; (2024) Fabrication of electrospun ion exchanger adsorbents with morphologies designed for the separation of proteins and plasmid DNA. Journal of Chromatography A , 1734 , Article 465268. 10.1016/j.chroma.2024.465268 <https://doi.org/10.1016/j.chroma.2024.465268>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10198355/1/1-s2.0-S0021967324006423-main.pdf