eprintid: 10197406
rev_number: 6
eprint_status: archive
userid: 699
dir: disk0/10/19/74/06
datestamp: 2024-09-25 08:36:19
lastmod: 2024-09-25 08:36:19
status_changed: 2024-09-25 08:36:19
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Shire, Ebony
creators_name: Coimbra, André AB
creators_name: Barba Ostria, Carlos
creators_name: Rios-Solis, Leonardo
creators_name: López Barreiro, Diego
title: Molecular design of protein-based materials – state of the art, opportunities and challenges at the interface between materials engineering and synthetic biology
ispublished: inpress
divisions: UCL
divisions: B04
divisions: F43
note: © Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).
abstract: Structural proteins like silk, squid ring teeth, elastin, collagen, or resilin, among others, are inspiring the development of new sustainable biopolymeric materials for applications including healthcare, food, soft robotics, or textiles. Furthermore, advances in the fields of soft materials and synthetic biology have a joint great potential to guide the design of novel structural proteins, despite both fields progressing mostly in a separate fashion so far. Using recombinant DNA technologies and microbial fermentations, we can design new structural proteins with monomer-level sequence control and a dispersity of ca. 1.0, based on permutations of tandem repeats derived from natural structural proteins. However, the molecular design of recombinant and repetitive structural proteins is a nontrivial task that is generally approached using low-throughput trial-and-error experimentation. Here, we review recent progress in this area, in terms of structure–function relationships and DNA synthesis technologies. We also discuss experimental and computational advances towards the establishment of rapid prototyping pipelines for this family of biopolymers. Finally, we highlight future challenges to make protein-based materials a commercially viable alternative to current fossil-based polymers.
date: 2024-09-19
date_type: published
publisher: Royal Society of Chemistry (RSC)
official_url: http://dx.doi.org/10.1039/d4me00122b
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2314649
doi: 10.1039/d4me00122b
lyricists_name: Lopez Barreiro, Diego
lyricists_id: DLOPE24
actors_name: Lopez Barreiro, Diego
actors_id: DLOPE24
actors_role: owner
full_text_status: public
publication: Molecular Systems Design & Engineering
citation:        Shire, Ebony;    Coimbra, André AB;    Barba Ostria, Carlos;    Rios-Solis, Leonardo;    López Barreiro, Diego;      (2024)    Molecular design of protein-based materials – state of the art, opportunities and challenges at the interface between materials engineering and synthetic biology.                   Molecular Systems Design & Engineering        10.1039/d4me00122b <https://doi.org/10.1039/d4me00122b>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10197406/1/D4ME00122B.pdf