eprintid: 10197085
rev_number: 7
eprint_status: archive
userid: 699
dir: disk0/10/19/70/85
datestamp: 2024-09-17 10:57:00
lastmod: 2024-09-17 10:57:00
status_changed: 2024-09-17 10:57:00
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Giannoni, Luca
creators_name: Marradi, Marta
creators_name: Scibilia, Kevin
creators_name: Ezhov, Ivan
creators_name: Bonaudo, Camilla
creators_name: Artemiou, Angelos
creators_name: Toaha, Anam
creators_name: Lange, Frédéric
creators_name: Caredda, Charly
creators_name: Montcel, Bruno
creators_name: Puppa, Alessandro Della
creators_name: Tachtsidis, Ilias
creators_name: Rückert, Daniel
creators_name: Pavone, Francesco Saverio
title: Transportable hyperspectral imaging setup based on fast, high-density spectral scanning for in situ quantitative biochemical mapping of fresh tissue biopsies
ispublished: pub
divisions: UCL
divisions: B04
divisions: F42
keywords: Hyperspectral imaging; biomedical optics; biophotonics; digital histopathology; neurosurgery
note: Copyright © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License, https://creativecommons.org/licenses/by/4.0/deed.en.
Distribution or reproduction of this work in whole or in part requires full attribution of the original
publication, including its DOI. [DOI: 10.1117/1.JBO.29.9.093508]
abstract: Significance:
Histopathological examination of surgical biopsies, such as in glioma and glioblastoma resection, is hindered in current clinical practice by the long time required for the laboratory analysis and pathological screening, typically taking several days or even weeks to be completed.
//
Aim:
We propose here a transportable, high-density, spectral scanning-based hyperspectral imaging (HSI) setup, named HyperProbe1, that can provide in situ, fast biochemical analysis, and mapping of fresh surgical tissue samples, right after excision, and without the need for fixing, staining nor compromising the integrity of the tissue properties.
//
Approach:
HyperProbe1 is based on spectral scanning via supercontinuum laser illumination filtered with acousto-optic tunable filters. Such methodology allows the user to select any number and type of wavelength bands in the visible and near-infrared range between 510 and 900 nm (up to a maximum of 79) and to reconstruct 3D hypercubes composed of high-resolution (4 to 
5
μ
m
), widefield images (
0.9
×
0.9
mm
2
) of the surgical samples, where each pixel is associated with a complete spectrum.
//
Results:
The HyperProbe1 setup is here presented and characterized. The system is applied to 11 fresh surgical biopsies of glioma from routine patients, including different grades of tumor classification. Quantitative analysis of the composition of the tissue is performed via fast spectral unmixing to reconstruct the mapping of major biomarkers, such as oxy-(
HbO
2
) and deoxyhemoglobin (HHb), as well as cytochrome-c-oxidase (CCO). We also provided a preliminary attempt to infer tumor classification based on differences in composition in the samples, suggesting the possibility of using lipid content and differential CCO concentrations to distinguish between lower and higher-grade gliomas.
//
Conclusions:
A proof of concept of the performances of HyperProbe1 for quantitative, biochemical mapping of surgical biopsies is demonstrated, paving the way for improving current post-surgical, histopathological practice via non-destructive, in situ streamlined screening of fresh tissue samples in a matter of minutes after excision.
date: 2024-09
date_type: published
publisher: SPIE-Intl Soc Optical Eng
official_url: http://dx.doi.org/10.1117/1.jbo.29.9.093508
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2311991
doi: 10.1117/1.JBO.29.9.093508
medium: Print-Electronic
pii: 240139SSRR
lyricists_name: Lange, Frederic
lyricists_name: Tachtsidis, Ilias
lyricists_id: FLANG91
lyricists_id: ITACH19
actors_name: Lange, Frederic
actors_id: FLANG91
actors_role: owner
full_text_status: public
publication: Journal of Biomedical Optics
volume: 29
number: 9
article_number: 093508
event_location: United States
issn: 1083-3668
citation:        Giannoni, Luca;    Marradi, Marta;    Scibilia, Kevin;    Ezhov, Ivan;    Bonaudo, Camilla;    Artemiou, Angelos;    Toaha, Anam;                             ... Pavone, Francesco Saverio; + view all <#>        Giannoni, Luca;  Marradi, Marta;  Scibilia, Kevin;  Ezhov, Ivan;  Bonaudo, Camilla;  Artemiou, Angelos;  Toaha, Anam;  Lange, Frédéric;  Caredda, Charly;  Montcel, Bruno;  Puppa, Alessandro Della;  Tachtsidis, Ilias;  Rückert, Daniel;  Pavone, Francesco Saverio;   - view fewer <#>    (2024)    Transportable hyperspectral imaging setup based on fast, high-density spectral scanning for in situ quantitative biochemical mapping of fresh tissue biopsies.                   Journal of Biomedical Optics , 29  (9)    , Article 093508.  10.1117/1.JBO.29.9.093508 <https://doi.org/10.1117/1.JBO.29.9.093508>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10197085/1/093508_1.pdf