eprintid: 10196240
rev_number: 6
eprint_status: archive
userid: 699
dir: disk0/10/19/62/40
datestamp: 2024-08-29 08:51:21
lastmod: 2024-08-29 08:51:21
status_changed: 2024-08-29 08:51:21
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Chen, Jishizhan
creators_name: Birchall, Martin
creators_name: MacRobert, Alexander J
creators_name: Song, Wenhui
title: Liquid Crystalline Hydroxyapatite Nanorods Orchestrate Hierarchical Bone‐Like Mineralization
ispublished: inpress
divisions: UCL
divisions: B04
divisions: F45
keywords: hydroxyapatite nanorods, liquid crystalline structure, mineralization, osteogenesis, stem cells
note: © 2024 The Author(s). Small published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
abstract: Bone matrix exhibits exceptional mechanical properties due to its unique nanocomposite structure of type I collagen fibrils and hydroxyapatite (HAp) nanoparticles in hierarchical liquid crystalline (LC) order. However, the regeneration mechanism of this LC structure is elusive. This study investigates the role of the LC structure of HAp nanorods in guiding aligned mineralization and its underlying molecular mechanism. A unidirectionally oriented LC phase of HAp nanorods is developed through engineering‐assisted self‐assembling. This is used to study the growth direction of long‐range aligned extracellular matrix (ECM) and calcium deposit formation during the osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells. It is found that 2 key regulatory genes, COL1A1 and COL4A6, lead to the formation of aligned ECM. Activation of the PI3K‐Akt pathway enhances osteogenesis and promotes ordered calcium deposits. This study provides evidence for elucidating the mechanism of LC‐induced ordered calcium deposition at hierarchical levels spanning from the molecular to macro‐scale, as well as the switch from ordered to disordered mineralization. These findings illuminate bone regeneration, contribute to the development of biomimetic artificial bone with long‐range ordered structures, and suggest a basis for therapeutic targeting of microstructure‐affected bone disorders and the broader field of cell‐ECM interactions.
date: 2024-08-23
date_type: published
publisher: Wiley
official_url: http://dx.doi.org/10.1002/smll.202310024
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2307433
doi: 10.1002/smll.202310024
lyricists_name: Chen, Jishizhan
lyricists_id: JCHEI07
actors_name: Chen, Jishizhan
actors_id: JCHEI07
actors_role: owner
full_text_status: public
publication: Small
citation:        Chen, Jishizhan;    Birchall, Martin;    MacRobert, Alexander J;    Song, Wenhui;      (2024)    Liquid Crystalline Hydroxyapatite Nanorods Orchestrate Hierarchical Bone‐Like Mineralization.                   Small        10.1002/smll.202310024 <https://doi.org/10.1002/smll.202310024>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10196240/1/Small.pdf