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<https://discovery.ucl.ac.uk/id/eprint/10195312> <http://purl.org/dc/terms/title> "The Invisible Trail: The Application of The Microbiome in Forensic Investigations"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10195312> <http://purl.org/ontology/bibo/abstract> "Soil, a complex and dynamic substrate, plays a crucial role in forensic investigations due to\r\nits ubiquitous nature and ability to transfer trace evidence. The distinct physical, chemical,\r\nand biological properties of soil vary significantly with location and depth, offering valuable\r\nclues for crime reconstruction. While traditional soil analysis methods have been employed\r\nin forensic science, soil microbiome analysis presents a novel and complementary\r\napproach. This study explores the potential of utilising a 16S rRNA amplicon sequencing to\r\ncharacterise the soil microbiome, offering a digital signature for distinguishing soil samples\r\nand aiding in criminal investigations.\r\nThe findings show that microbial communities differ significantly between surface and subsurface\r\nsoils, indicating depth as a strong discriminating factor within a single site.\r\nMoreover, variations in microbial profiles allow for inferences to be made regarding the\r\norigin of soil samples, distinguishing between surficial soils which can be transferred onto\r\nfootwear from walking, with soils from depth at a grave site.\r\nAcross multiple sites, location emerges as a stronger differentiating factor than depth,\r\nenabling discrimination between samples even within close proximity (less than 1km).\r\nNotably, the microbiome proves valuable in distinguishing soils of the same or differing\r\nclassifications.\r\nDNA concentration analysis complements microbiome profiling in determining soil depth.\r\nInformative microbial profiles can be generated from nanogram DNA concentrations,\r\nhighlighting the sensitivity of the method. However, these methods are susceptible to\r\nsequencing bias and careful consideration should given to experimental design, sample\r\ncollection and analysis pipeline.\r\nFrom the findings from the studies undertaken in this thesis it was possible to conclude that\r\nsoil microbial communities vary with depth and distance; DNA concentration aids in\r\ndetermining soil depth; microbial profiles can be obtained from minimal DNA amounts;\r\nclose-proximity samples can be differentiated; and each soil context warrants independent\r\nassessment.\r\n\r\nOverall, this research demonstrates the promising potential of soil microbiome analysis in\r\nforensic investigations, offering a valuable tool for discriminating soil samples based on\r\ndepth and location."^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10195312> <http://purl.org/dc/terms/date> "2024-07-28" .
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