eprintid: 10194411 rev_number: 8 eprint_status: archive userid: 699 dir: disk0/10/19/44/11 datestamp: 2024-07-11 07:29:37 lastmod: 2024-07-11 07:29:37 status_changed: 2024-07-11 07:29:37 type: article metadata_visibility: show sword_depositor: 699 creators_name: Sawadkar, Prasad creators_name: Mandakhbayar, Nandin creators_name: Patel, Kapil D creators_name: Owji, Nazanin creators_name: Rajasekar, Poojitha creators_name: Sarama, Roudin creators_name: Lee, Jung-Hwan creators_name: Kim, Hae-Won creators_name: Knowles, Jonathan creators_name: Garcia-Gareta, Elena title: 3D Porous Binary Composites of Collagen, Elastin, and Fibrin Proteins Orchestrate Adipose Tissue Regeneration ispublished: inpress divisions: UCL divisions: B02 divisions: B04 divisions: C10 divisions: C05 divisions: D16 divisions: D21 divisions: F47 divisions: G88 divisions: H10 keywords: Adipose tissue engineering; ADSCs; fat regeneration; stem cells note: Copyright © 2024 The Author(s). Macromolecular Bioscience published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, https://creativecommons.org/licenses/by/4.0/, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. abstract: The objective for this study is to advance the development of a specialized biomaterial that can effectively facilitate the regeneration of adipose tissue. In prior studies, the assessment of collagen (Col), elastin (Ela), and fibrin (Fib) unary scaffolds has been conducted. However, it is important to note that native adipose tissue is comprised of a diverse array of extracellular matrix (ECM) constituents. To mimic this behavior, binary compositions of collagen, elastin, and fibrin are fabricated in a 1:1 ratio, resulting in the formation of Col/Ela, Col/Fib, and Ela/Fib composites through a customized fabrication procedure. The physical properties of these scaffolds are comprehensively analyzed using a range of material characterization techniques. Additionally, the biological properties of the scaffolds are investigated by examining the survival, proliferation, and phenotype of adipose-derived stem cells. Subsequently, the aforementioned binary scaffolds are implanted into a rodent model for 28 days. the explants are analysed through X-ray microtomography, histology, and immunohistochemistry. The findings of the study demonstrate that the utilization of binary combinations of Col/Ela, Col/Fib, and Ela/Fib has a discernible impact on the physical and biological characteristics of the scaffolds. Nevertheless, Ela/Fib exhibits characteristics that make it a suitable candidate for adipogenesis due to its notable upregulation of caveolin-1 expression in both acellular and cellular cohorts. The combination of two natural polymers in this cell–material interaction has significantly enhanced the comprehension of adipogenesis. date: 2024-06-06 date_type: published publisher: WILEY-V C H VERLAG GMBH official_url: http://dx.doi.org/10.1002/mabi.202400073 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2282293 doi: 10.1002/mabi.202400073 medium: Print-Electronic lyricists_name: Knowles, Jonathan lyricists_name: Sawadkar, Prasad lyricists_name: Owji, Nazanin lyricists_id: JCKNO52 lyricists_id: PSAWA87 lyricists_id: NOWJI02 actors_name: Sawadkar, Prasad actors_id: PSAWA87 actors_role: owner funding_acknowledgements: 299811 [Restoration of Appearance and Function Trust]; 2018K1A4A3A01064257 [Restoration of Appearance and Function Trust (UK Registered Charity)]; 2019R1A6A1A11034536 [National Research Foundation (NRF) of Republic of Korea]; 2021R1A5A2022318 [Priority Research Centre Program]; 2018R1D1A1B07048020 [Medical Research Centre Program]; RYC2021-033490-I [Basic science program]; MCIN/AEI/10.13039/501100011033 [Basic science program]; [Ministry of Education or Ministry of Science and Technology, Republic of Korea - Ramon & Cajal Fellowship]; [EU] full_text_status: public publication: Macromolecular Bioscience article_number: 2400073 pages: 12 event_location: Germany issn: 1616-5187 citation: Sawadkar, Prasad; Mandakhbayar, Nandin; Patel, Kapil D; Owji, Nazanin; Rajasekar, Poojitha; Sarama, Roudin; Lee, Jung-Hwan; ... Garcia-Gareta, Elena; + view all <#> Sawadkar, Prasad; Mandakhbayar, Nandin; Patel, Kapil D; Owji, Nazanin; Rajasekar, Poojitha; Sarama, Roudin; Lee, Jung-Hwan; Kim, Hae-Won; Knowles, Jonathan; Garcia-Gareta, Elena; - view fewer <#> (2024) 3D Porous Binary Composites of Collagen, Elastin, and Fibrin Proteins Orchestrate Adipose Tissue Regeneration. Macromolecular Bioscience , Article 2400073. 10.1002/mabi.202400073 <https://doi.org/10.1002/mabi.202400073>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10194411/1/Macromolecular%20Bioscience%20-%202024%20-%20Sawadkar%20-%203D%20Porous%20Binary%20Composites%20of%20Collagen%20Elastin%20and%20Fibrin%20Proteins-1.pdf