eprintid: 10193544
rev_number: 7
eprint_status: archive
userid: 699
dir: disk0/10/19/35/44
datestamp: 2024-06-18 09:04:28
lastmod: 2024-06-18 09:04:28
status_changed: 2024-06-18 09:04:28
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Fakhrudin, Nanang
creators_name: Pertiwi, Krisna Kharisma
creators_name: Takubessi, Marce Inggrita
creators_name: Susiani, Eka Fitri
creators_name: Nurrochmad, Arief
creators_name: Widyarini, Sitarina
creators_name: Sudarmanto, Ari
creators_name: Nugroho, Arief Adi
creators_name: Wahyuono, Subagus
title: A geranylated chalcone with antiplatelet activity from the leaves of breadfruit (Artocarpus altilis)
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
keywords: Artocarpus communis, 2-geranyl-2ʹ,3,3,4ʹ-tetrahydroxydihydrochalcone, thrombosis, P2Y12, P2Y1
note: Copyright Fakhrudin N et al. This is an open access article distributed under the terms of the Creative Commons Attribution License
(CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source
are credited.
abstract: Platelet plays a crucial role in cardiovascular diseases (CVDs) development. Abnormalities in platelet aggregation provokes thromboembolism, eventually leading to death. In Indonesia, breadfruit (Artocarpus altilis) leaf is traditionally used to treat CVDs. This study aimed to evaluate the antiplatelet activity of A. altilis leaf extract (AAE) and to identify its active compound. A. altilis leaves were extracted with ethanol, and the antiplatelet activity was assessed using ADP-induced platelet aggregation. The major compound was isolated with column chromatography followed by preparative TLC, and the structure was determined on the basis of UV, MS, IR, and NMR spectra. The binding mode of the active compound to platelet receptors was characterized in in silico study. AAE exhibited an antiplatelet activity (IC50 of 252.23 µg/mL). A geranylated chalcone, 2-geranyl-2ʹ,3,3,4ʹ-tetrahydroxydihydrochalcone (GTDC) was identified as the antiplatelet compound (IC50 of 9.09 µM). GTDC actions with P2Y12 platelet receptor involving three amino acid residues.
date: 2020-10-02
date_type: published
publisher: Pensoft Publishers
official_url: http://dx.doi.org/10.3897/pharmacia.67.e56788
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2286946
doi: 10.3897/pharmacia.67.e56788
lyricists_name: Bessi, Marce
lyricists_id: MITAK82
actors_name: Bessi, Marce
actors_id: MITAK82
actors_role: owner
full_text_status: public
publication: Pharmacia
volume: 67
number: 4
pagerange: 173-180
issn: 0428-0296
citation:        Fakhrudin, Nanang;    Pertiwi, Krisna Kharisma;    Takubessi, Marce Inggrita;    Susiani, Eka Fitri;    Nurrochmad, Arief;    Widyarini, Sitarina;    Sudarmanto, Ari;         ... Wahyuono, Subagus; + view all <#>        Fakhrudin, Nanang;  Pertiwi, Krisna Kharisma;  Takubessi, Marce Inggrita;  Susiani, Eka Fitri;  Nurrochmad, Arief;  Widyarini, Sitarina;  Sudarmanto, Ari;  Nugroho, Arief Adi;  Wahyuono, Subagus;   - view fewer <#>    (2020)    A geranylated chalcone with antiplatelet activity from the leaves of breadfruit (Artocarpus altilis).                   Pharmacia , 67  (4)   pp. 173-180.    10.3897/pharmacia.67.e56788 <https://doi.org/10.3897/pharmacia.67.e56788>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10193544/1/PHAR_article_56788_en_1.pdf