TY  - JOUR
TI  - A geranylated chalcone with antiplatelet activity from the leaves of breadfruit (Artocarpus altilis)
AV  - public
Y1  - 2020/10/02/
EP  - 180
N2  - Platelet plays a crucial role in cardiovascular diseases (CVDs) development. Abnormalities in platelet aggregation provokes thromboembolism, eventually leading to death. In Indonesia, breadfruit (Artocarpus altilis) leaf is traditionally used to treat CVDs. This study aimed to evaluate the antiplatelet activity of A. altilis leaf extract (AAE) and to identify its active compound. A. altilis leaves were extracted with ethanol, and the antiplatelet activity was assessed using ADP-induced platelet aggregation. The major compound was isolated with column chromatography followed by preparative TLC, and the structure was determined on the basis of UV, MS, IR, and NMR spectra. The binding mode of the active compound to platelet receptors was characterized in in silico study. AAE exhibited an antiplatelet activity (IC50 of 252.23 痢/mL). A geranylated chalcone, 2-geranyl-2?,3,3,4?-tetrahydroxydihydrochalcone (GTDC) was identified as the antiplatelet compound (IC50 of 9.09 然). GTDC actions with P2Y12 platelet receptor involving three amino acid residues.
ID  - discovery10193544
PB  - Pensoft Publishers
KW  - Artocarpus communis
KW  -  2-geranyl-2?
KW  - 3
KW  - 3
KW  - 4?-tetrahydroxydihydrochalcone
KW  -  thrombosis
KW  -  P2Y12
KW  -  P2Y1
VL  - 67
SP  - 173
IS  - 4
N1  - Copyright Fakhrudin N et al. This is an open access article distributed under the terms of the Creative Commons Attribution License
(CC-BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source
are credited.
UR  - http://dx.doi.org/10.3897/pharmacia.67.e56788
SN  - 0428-0296
JF  - Pharmacia
A1  - Fakhrudin, Nanang
A1  - Pertiwi, Krisna Kharisma
A1  - Takubessi, Marce Inggrita
A1  - Susiani, Eka Fitri
A1  - Nurrochmad, Arief
A1  - Widyarini, Sitarina
A1  - Sudarmanto, Ari
A1  - Nugroho, Arief Adi
A1  - Wahyuono, Subagus
ER  -