eprintid: 10193493 rev_number: 12 eprint_status: archive userid: 699 dir: disk0/10/19/34/93 datestamp: 2024-06-14 10:38:17 lastmod: 2024-11-08 13:39:42 status_changed: 2024-06-14 10:38:17 type: article metadata_visibility: show sword_depositor: 699 creators_name: Salinger, Matthew T creators_name: Castellano Garrido, Daniel creators_name: Lamming, Eleanor D creators_name: Ward, John M creators_name: Moody, Thomas S creators_name: Jeffries, Jack WE creators_name: Hailes, Helen C title: An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: C06 divisions: F47 divisions: F56 keywords: biocatalysis, methyltransferases, methylation, cascade, catechols note: © 2024 The Authors. ChemCatChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). abstract: Methyltransferases are gaining traction as a method to achieve greener and regioselective methylations of a wide array of substrates. In this work, a halide methyltransferase biomethylation cascade was successfully adapted with the incorporation of a methionine adenosyltransferase to generate the S‐adenosyl‐L‐methionine (SAM) methylation cofactor in situ from less costly ATP and L‐methionine, instead of directly adding SAM to start the cascade. Furthermore, this cascade was applied to achieve novel catechol and tetrahydroisoquinoline methylations with the O‐methyltransferases RnCOMT, MxSafC and NpN4OMT with high conversions and regioselectivities. In addition, the cascade was successfully trialled with the less toxic methyl group donor, methyl tosylate. date: 2024-09-09 date_type: published publisher: Wiley official_url: http://dx.doi.org/10.1002/cctc.202400492 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2285219 doi: 10.1002/cctc.202400492 lyricists_name: Hailes, Helen lyricists_name: Ward, John lyricists_name: Jeffries, Jack lyricists_id: HCHAI24 lyricists_id: JMWAR05 lyricists_id: JEFFR95 actors_name: Hailes, Helen actors_id: HCHAI24 actors_role: owner full_text_status: public publication: ChemCatChem volume: 16 number: 7 article_number: e202400492 citation: Salinger, Matthew T; Castellano Garrido, Daniel; Lamming, Eleanor D; Ward, John M; Moody, Thomas S; Jeffries, Jack WE; Hailes, Helen C; (2024) An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases. ChemCatChem , 16 (7) , Article e202400492. 10.1002/cctc.202400492 <https://doi.org/10.1002/cctc.202400492>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10193493/7/Hailes_An%20Alternative%20Cascade%20for%20the%20Selective%20Methylation%20of%20Catechols%20and%20Tetrahydroisoquinolines%20by%20O-Methyltransferases_VoR.pdf