eprintid: 10193493
rev_number: 12
eprint_status: archive
userid: 699
dir: disk0/10/19/34/93
datestamp: 2024-06-14 10:38:17
lastmod: 2024-11-08 13:39:42
status_changed: 2024-06-14 10:38:17
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Salinger, Matthew T
creators_name: Castellano Garrido, Daniel
creators_name: Lamming, Eleanor D
creators_name: Ward, John M
creators_name: Moody, Thomas S
creators_name: Jeffries, Jack WE
creators_name: Hailes, Helen C
title: An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: C06
divisions: F47
divisions: F56
keywords: biocatalysis, methyltransferases, methylation, cascade, catechols
note: © 2024 The Authors. ChemCatChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
abstract: Methyltransferases are gaining traction as a method to achieve greener and regioselective methylations of a wide array of substrates. In this work, a halide methyltransferase biomethylation cascade was successfully adapted with the incorporation of a methionine adenosyltransferase to generate the S‐adenosyl‐L‐methionine (SAM) methylation cofactor in situ from less costly ATP and L‐methionine, instead of directly adding SAM to start the cascade. Furthermore, this cascade was applied to achieve novel catechol and tetrahydroisoquinoline methylations with the O‐methyltransferases RnCOMT, MxSafC and NpN4OMT with high conversions and regioselectivities. In addition, the cascade was successfully trialled with the less toxic methyl group donor, methyl tosylate.
date: 2024-09-09
date_type: published
publisher: Wiley
official_url: http://dx.doi.org/10.1002/cctc.202400492
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2285219
doi: 10.1002/cctc.202400492
lyricists_name: Hailes, Helen
lyricists_name: Ward, John
lyricists_name: Jeffries, Jack
lyricists_id: HCHAI24
lyricists_id: JMWAR05
lyricists_id: JEFFR95
actors_name: Hailes, Helen
actors_id: HCHAI24
actors_role: owner
full_text_status: public
publication: ChemCatChem
volume: 16
number: 7
article_number: e202400492
citation:        Salinger, Matthew T;    Castellano Garrido, Daniel;    Lamming, Eleanor D;    Ward, John M;    Moody, Thomas S;    Jeffries, Jack WE;    Hailes, Helen C;      (2024)    An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases.                   ChemCatChem , 16  (7)    , Article e202400492.  10.1002/cctc.202400492 <https://doi.org/10.1002/cctc.202400492>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10193493/7/Hailes_An%20Alternative%20Cascade%20for%20the%20Selective%20Methylation%20of%20Catechols%20and%20Tetrahydroisoquinolines%20by%20O-Methyltransferases_VoR.pdf