@article{discovery10193493, publisher = {Wiley}, volume = {16}, month = {September}, year = {2024}, title = {An Alternative Cascade for the Selective Methylation of Catechols and Tetrahydroisoquinolines by O-Methyltransferases}, number = {7}, journal = {ChemCatChem}, note = {{\copyright} 2024 The Authors. ChemCatChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).}, url = {http://dx.doi.org/10.1002/cctc.202400492}, abstract = {Methyltransferases are gaining traction as a method to achieve greener and regioselective methylations of a wide array of substrates. In this work, a halide methyltransferase biomethylation cascade was successfully adapted with the incorporation of a methionine adenosyltransferase to generate the S-adenosyl-L-methionine (SAM) methylation cofactor in situ from less costly ATP and L-methionine, instead of directly adding SAM to start the cascade. Furthermore, this cascade was applied to achieve novel catechol and tetrahydroisoquinoline methylations with the O-methyltransferases RnCOMT, MxSafC and NpN4OMT with high conversions and regioselectivities. In addition, the cascade was successfully trialled with the less toxic methyl group donor, methyl tosylate.}, author = {Salinger, Matthew T and Castellano Garrido, Daniel and Lamming, Eleanor D and Ward, John M and Moody, Thomas S and Jeffries, Jack WE and Hailes, Helen C}, keywords = {biocatalysis, methyltransferases, methylation, cascade, catechols} }