<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10191588> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/title> "Integrated micro-scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/ontology/bibo/abstract> "High throughput process development (HTPD) is established for time- and resource- efficient chromatographic process development. However, integration with non-chromatographic operations within a monoclonal antibody (mAb) purification train is less developed. An area of importance is the development of low pH viral inactivation (VI) that follows protein A chromatography. However, the lack of pH measurement devices at the micro-scale represents a barrier to implementation, which prevents integration with the surrounding unit operations, limiting overall process knowledge. This study is based upon the design and testing of a HTPD platform for integration of the protein A and low pH VI operations. This was achieved by using a design and simulation software before execution on an automated liquid handler. The operations were successfully translated to the micro-scale, as assessed by analysis of recoveries and molecular weight content. The integrated platform was then used as a tool to assess the effect of pH on HMWC during low pH hold. The laboratory-scale and micro-scale elution pools showed comparable HMWC across the pH range 3.2-3.7. The investigative power of the platform is highlighted by evaluating the resources required to conduct a hypothetical experiment. This results in lower resource demands and increased labor efficiency relative to the laboratory-scale. For example, the experiment can be conducted in 7 h, compared to 105 h, translating to labor hours, 3 h and 28 h for the micro-scale and laboratory-scale, respectively. This presents the opportunity for further integration beyond chromatographic operations within the purification sequence, to establish a fit-to-platform assessment tool for mAb process development."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/date> "2024-04-30" . <https://discovery.ucl.ac.uk/id/document/1738809> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/org/ext-e7795bc59c66fd1972aa29b4459edb20> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> . <https://discovery.ucl.ac.uk/id/org/ext-e7795bc59c66fd1972aa29b4459edb20> <http://xmlns.com/foaf/0.1/name> "Wiley"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/publisher> <https://discovery.ucl.ac.uk/id/org/ext-e7795bc59c66fd1972aa29b4459edb20> . <https://discovery.ucl.ac.uk/id/publication/ext-87567938> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> . <https://discovery.ucl.ac.uk/id/publication/ext-87567938> <http://xmlns.com/foaf/0.1/name> "Biotechnology Progress"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-87567938> . <https://discovery.ucl.ac.uk/id/publication/ext-87567938> <http://www.w3.org/2002/07/owl#sameAs> <urn:issn:87567938> . <https://discovery.ucl.ac.uk/id/publication/ext-87567938> <http://purl.org/ontology/bibo/issn> "87567938" . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-47e0064a49db9037c88ab31db8360676> . 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<https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> . <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-456b076fccb273e65a383cb9721330c4> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> . <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> . <https://discovery.ucl.ac.uk/id/eprint/10191588#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> . <https://discovery.ucl.ac.uk/id/person/ext-f514518bb161e3055953d7e10c6518e3> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-f514518bb161e3055953d7e10c6518e3> <http://xmlns.com/foaf/0.1/givenName> "Lars"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f514518bb161e3055953d7e10c6518e3> <http://xmlns.com/foaf/0.1/familyName> "Robbel"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f514518bb161e3055953d7e10c6518e3> <http://xmlns.com/foaf/0.1/name> "Lars Robbel"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-47e0064a49db9037c88ab31db8360676> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-47e0064a49db9037c88ab31db8360676> <http://xmlns.com/foaf/0.1/givenName> "Paras"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-47e0064a49db9037c88ab31db8360676> <http://xmlns.com/foaf/0.1/familyName> "Sharma"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-47e0064a49db9037c88ab31db8360676> <http://xmlns.com/foaf/0.1/name> "Paras Sharma"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> <http://xmlns.com/foaf/0.1/givenName> "Duygu"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> <http://xmlns.com/foaf/0.1/familyName> "Dikicioglu"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8fd08996a0a598f39869404a8920611c> <http://xmlns.com/foaf/0.1/name> "Duygu Dikicioglu"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> <http://xmlns.com/foaf/0.1/givenName> "Daniel G"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> <http://xmlns.com/foaf/0.1/familyName> "Bracewell"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-94bbc64074a2f7160e89e255f084917b> <http://xmlns.com/foaf/0.1/name> "Daniel G Bracewell"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-456b076fccb273e65a383cb9721330c4> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-456b076fccb273e65a383cb9721330c4> <http://xmlns.com/foaf/0.1/givenName> "Michael"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-456b076fccb273e65a383cb9721330c4> <http://xmlns.com/foaf/0.1/familyName> "Schmitt"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-456b076fccb273e65a383cb9721330c4> <http://xmlns.com/foaf/0.1/name> "Michael Schmitt"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10191588> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . 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