eprintid: 10190622
rev_number: 9
eprint_status: archive
userid: 699
dir: disk0/10/19/06/22
datestamp: 2024-04-12 13:41:42
lastmod: 2024-04-12 13:41:42
status_changed: 2024-04-12 13:41:42
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Jiang, W
creators_name: Cai, Y
creators_name: Sun, S
creators_name: Wang, W
creators_name: Tišma, M
creators_name: Baganz, F
creators_name: Hao, J
title: Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F47
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Klebsiella pneumoniae can use glucose or glycerol as carbon sources to produce 1,3-propanediol or 2,3-butanediol, respectively. In the metabolism of Klebsiella pneumoniae, hydrogenase-3 is responsible for H2 production from formic acid, but it is not directly related to the synthesis pathways for 1,3-propanediol and 2,3-butanediol. In the first part of this research, hycEFG, which encodes subunits of the enzyme hydrogenase-3, was knocked out, so K. pneumoniae ΔhycEFG lost the ability to produce H2 during cultivation using glycerol as a carbon source. As a consequence, the concentration of 1,3-propanediol increased and the substrate (glycerol) conversion ratio reached 0.587 mol/mol. Then, K. pneumoniae ΔldhAΔhycEFG was constructed to erase lactic acid synthesis which led to the further increase of 1,3-propanediol concentration. A substrate (glycerol) conversion ratio of 0.628 mol/mol in batch conditions was achieved, which was higher compared to the wild type strain (0.545 mol/mol). Furthermore, since adhE encodes an alcohol dehydrogenase that catalyzes ethanol production from acetaldehyde, K. pneumoniae ΔldhAΔadhEΔhycEFG was constructed to prevent ethanol production. Contrary to expectations, this did not lead to a further increase, but to a decrease in 1,3-propanediol production. In the second part of this research, glucose was used as the carbon source to produce 2,3-butanediol. Knocking out hycEFG had distinct positive effect on 2,3-butanediol production. Especially in K. pneumoniae ΔldhAΔadhEΔhycEFG, a substrate (glucose) conversion ratio of 0.730 mol/mol was reached, which is higher compared to wild type strain (0.504 mol/mol). This work suggests that the inactivation of hydrogenase-3 may have a global effect on the metabolic regulation of K. pneumoniae, leading to the improvement of the production of two industrially important bulk chemicals, 1,3-propanediol and 2,3-butanediol.
date: 2024-06
date_type: published
publisher: Elsevier BV
official_url: https://doi.org/10.1016/j.enzmictec.2024.110438
full_text_type: other
language: eng
verified: verified_manual
elements_id: 2263495
doi: 10.1016/j.enzmictec.2024.110438
medium: Print-Electronic
pii: S0141-0229(24)00045-0
lyricists_name: Baganz, Frank
lyricists_id: FBAGA65
actors_name: Baganz, Frank
actors_id: FBAGA65
actors_role: owner
full_text_status: restricted
publication: Enzyme and Microbial Technology
volume: 177
article_number: 110438
event_location: United States
issn: 0141-0229
citation:        Jiang, W;    Cai, Y;    Sun, S;    Wang, W;    Tišma, M;    Baganz, F;    Hao, J;      (2024)    Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae.                   Enzyme and Microbial Technology , 177     , Article 110438.  10.1016/j.enzmictec.2024.110438 <https://doi.org/10.1016/j.enzmictec.2024.110438>.      
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10190622/1/Baganz_Inactivation%20of%20hydrogenase%20MS_Final.pdf