<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10190622> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/title> "Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/abstract> "Klebsiella pneumoniae can use glucose or glycerol as carbon sources to produce 1,3-propanediol or 2,3-butanediol, respectively. In the metabolism of Klebsiella pneumoniae, hydrogenase-3 is responsible for H2 production from formic acid, but it is not directly related to the synthesis pathways for 1,3-propanediol and 2,3-butanediol. In the first part of this research, hycEFG, which encodes subunits of the enzyme hydrogenase-3, was knocked out, so K. pneumoniae ΔhycEFG lost the ability to produce H2 during cultivation using glycerol as a carbon source. As a consequence, the concentration of 1,3-propanediol increased and the substrate (glycerol) conversion ratio reached 0.587 mol/mol. Then, K. pneumoniae ΔldhAΔhycEFG was constructed to erase lactic acid synthesis which led to the further increase of 1,3-propanediol concentration. A substrate (glycerol) conversion ratio of 0.628 mol/mol in batch conditions was achieved, which was higher compared to the wild type strain (0.545 mol/mol). Furthermore, since adhE encodes an alcohol dehydrogenase that catalyzes ethanol production from acetaldehyde, K. pneumoniae ΔldhAΔadhEΔhycEFG was constructed to prevent ethanol production. Contrary to expectations, this did not lead to a further increase, but to a decrease in 1,3-propanediol production. In the second part of this research, glucose was used as the carbon source to produce 2,3-butanediol. Knocking out hycEFG had distinct positive effect on 2,3-butanediol production. Especially in K. pneumoniae ΔldhAΔadhEΔhycEFG, a substrate (glucose) conversion ratio of 0.730 mol/mol was reached, which is higher compared to wild type strain (0.504 mol/mol). This work suggests that the inactivation of hydrogenase-3 may have a global effect on the metabolic regulation of K. pneumoniae, leading to the improvement of the production of two industrially important bulk chemicals, 1,3-propanediol and 2,3-butanediol."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/date> "2024-06" . <https://discovery.ucl.ac.uk/id/document/1718843> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/volume> "177" . <https://discovery.ucl.ac.uk/id/org/ext-464ff5f0fac4572c5372d3e7fc978be1> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> . <https://discovery.ucl.ac.uk/id/org/ext-464ff5f0fac4572c5372d3e7fc978be1> <http://xmlns.com/foaf/0.1/name> "Elsevier BV"^^<http://www.w3.org/2001/XMLSchema#string> . 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<https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> . <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> . <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> . <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> . <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> . <https://discovery.ucl.ac.uk/id/eprint/10190622#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_7> <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> . <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> <http://xmlns.com/foaf/0.1/givenName> "S"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> <http://xmlns.com/foaf/0.1/familyName> "Sun"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-b9485dc33d6a643f08f9ac73cbfd2410> <http://xmlns.com/foaf/0.1/name> "S Sun"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> <http://xmlns.com/foaf/0.1/givenName> "M"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> <http://xmlns.com/foaf/0.1/familyName> "Tišma"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-1bcee45bbfa97f6e9a506910e7084dc9> <http://xmlns.com/foaf/0.1/name> "M Tišma"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> <http://xmlns.com/foaf/0.1/givenName> "W"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> <http://xmlns.com/foaf/0.1/familyName> "Wang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-4a86a821ab7c7da06c0ff7710b0d502d> <http://xmlns.com/foaf/0.1/name> "W Wang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-78572e90dff834be51beb741ef46f720> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-78572e90dff834be51beb741ef46f720> <http://xmlns.com/foaf/0.1/givenName> "Y"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-78572e90dff834be51beb741ef46f720> <http://xmlns.com/foaf/0.1/familyName> "Cai"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-78572e90dff834be51beb741ef46f720> <http://xmlns.com/foaf/0.1/name> "Y Cai"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> <http://xmlns.com/foaf/0.1/givenName> "F"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> <http://xmlns.com/foaf/0.1/familyName> "Baganz"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-b5cf43ec5284de9901a022b4a0ab7cd4> <http://xmlns.com/foaf/0.1/name> "F Baganz"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8dc3bf90256e59a28ed807626303b850> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-8dc3bf90256e59a28ed807626303b850> <http://xmlns.com/foaf/0.1/givenName> "W"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8dc3bf90256e59a28ed807626303b850> <http://xmlns.com/foaf/0.1/familyName> "Jiang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-8dc3bf90256e59a28ed807626303b850> <http://xmlns.com/foaf/0.1/name> "W Jiang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> <http://xmlns.com/foaf/0.1/givenName> "J"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> <http://xmlns.com/foaf/0.1/familyName> "Hao"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-14e41b537259b2015d1574184683f28a> <http://xmlns.com/foaf/0.1/name> "J Hao"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1718843> . <https://discovery.ucl.ac.uk/id/document/1718843> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1718843> <http://www.w3.org/2000/01/rdf-schema#label> "Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae (Text)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10190622> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1724372> . <https://discovery.ucl.ac.uk/id/document/1724372> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1724372> <http://www.w3.org/2000/01/rdf-schema#label> "Inactivation of hydrogenase-3 leads to enhancement of 1,3-propanediol and 2,3-butanediol production by Klebsiella pneumoniae (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1724372> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1718843> . <https://discovery.ucl.ac.uk/id/document/1724372> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1718843> . 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