eprintid: 10190381 rev_number: 8 eprint_status: archive userid: 699 dir: disk0/10/19/03/81 datestamp: 2024-04-09 15:08:18 lastmod: 2024-09-07 06:10:08 status_changed: 2024-04-09 15:08:18 type: article metadata_visibility: show sword_depositor: 699 creators_name: Dorey, Adam creators_name: Howorka, Stefan title: Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics ispublished: pub divisions: UCL divisions: B04 divisions: C06 divisions: F56 keywords: Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, REAL-TIME, BIOLOGICAL NANOPORE, ALPHA-HEMOLYSIN, LIPID-BILAYER, MSPA NANOPORE, HUMAN GENOME, 3 DECADES, PROTEIN, MEMBRANE, TRANSLOCATION note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores also show promise for the technologically even more challenging sequencing of polypeptides, something that could considerably benefit biological discovery, clinical diagnostics and homeland security, as current techniques lack portability and speed. Here we survey the biochemical innovations underpinning commercial and academic nanopore DNA/RNA sequencing techniques, and explore how these advances can fuel developments in future protein sequencing with nanopores. date: 2024-03-06 date_type: published publisher: Nature Publishing Group official_url: http://dx.doi.org/10.1038/s41557-023-01322-x oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2260457 doi: 10.1038/s41557-023-01322-x medium: Print-Electronic pii: 10.1038/s41557-023-01322-x lyricists_name: Howorka, Stefan lyricists_id: SHOWO39 actors_name: Howorka, Stefan actors_id: SHOWO39 actors_role: owner funding_acknowledgements: RGP0047/2020 [Human Frontier Science Program (HFSP)]; EP/N009282/1 [RCUK | Engineering and Physical Sciences Research Council (EPSRC)]; EP/V02874X/1 [RCUK | Engineering and Physical Sciences Research Council (EPSRC)] full_text_status: public publication: Nature Chemistry volume: 16 pagerange: 314-334 event_location: England issn: 1755-4330 citation: Dorey, Adam; Howorka, Stefan; (2024) Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics. Nature Chemistry , 16 pp. 314-334. 10.1038/s41557-023-01322-x <https://doi.org/10.1038/s41557-023-01322-x>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10190381/1/NCHEM-deposited%20version%20no%20galley%20proof%20edits.pdf