eprintid: 10190381
rev_number: 8
eprint_status: archive
userid: 699
dir: disk0/10/19/03/81
datestamp: 2024-04-09 15:08:18
lastmod: 2024-09-07 06:10:08
status_changed: 2024-04-09 15:08:18
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Dorey, Adam
creators_name: Howorka, Stefan
title: Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F56
keywords: Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, REAL-TIME, BIOLOGICAL NANOPORE, ALPHA-HEMOLYSIN, LIPID-BILAYER, MSPA NANOPORE, HUMAN GENOME, 3 DECADES, PROTEIN, MEMBRANE, TRANSLOCATION
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores also show promise for the technologically even more challenging sequencing of polypeptides, something that could considerably benefit biological discovery, clinical diagnostics and homeland security, as current techniques lack portability and speed. Here we survey the biochemical innovations underpinning commercial and academic nanopore DNA/RNA sequencing techniques, and explore how these advances can fuel developments in future protein sequencing with nanopores.
date: 2024-03-06
date_type: published
publisher: Nature Publishing Group
official_url: http://dx.doi.org/10.1038/s41557-023-01322-x
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2260457
doi: 10.1038/s41557-023-01322-x
medium: Print-Electronic
pii: 10.1038/s41557-023-01322-x
lyricists_name: Howorka, Stefan
lyricists_id: SHOWO39
actors_name: Howorka, Stefan
actors_id: SHOWO39
actors_role: owner
funding_acknowledgements: RGP0047/2020 [Human Frontier Science Program (HFSP)]; EP/N009282/1 [RCUK | Engineering and Physical Sciences Research Council (EPSRC)]; EP/V02874X/1 [RCUK | Engineering and Physical Sciences Research Council (EPSRC)]
full_text_status: public
publication: Nature Chemistry
volume: 16
pagerange: 314-334
event_location: England
issn: 1755-4330
citation:        Dorey, Adam;    Howorka, Stefan;      (2024)    Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics.                   Nature Chemistry , 16    pp. 314-334.    10.1038/s41557-023-01322-x <https://doi.org/10.1038/s41557-023-01322-x>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10190381/1/NCHEM-deposited%20version%20no%20galley%20proof%20edits.pdf