TY  - JOUR
PB  - Nature Publishing Group
A1  - Dorey, Adam
A1  - Howorka, Stefan
JF  - Nature Chemistry
VL  - 16
SP  - 314
N2  - Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores also show promise for the technologically even more challenging sequencing of polypeptides, something that could considerably benefit biological discovery, clinical diagnostics and homeland security, as current techniques lack portability and speed. Here we survey the biochemical innovations underpinning commercial and academic nanopore DNA/RNA sequencing techniques, and explore how these advances can fuel developments in future protein sequencing with nanopores.
ID  - discovery10190381
UR  - http://dx.doi.org/10.1038/s41557-023-01322-x
Y1  - 2024/03/06/
KW  - Science & Technology
KW  -  Physical Sciences
KW  -  Chemistry
KW  -  Multidisciplinary
KW  -  Chemistry
KW  -  REAL-TIME
KW  -  BIOLOGICAL NANOPORE
KW  -  ALPHA-HEMOLYSIN
KW  -  LIPID-BILAYER
KW  -  MSPA NANOPORE
KW  -  HUMAN GENOME
KW  -  3 DECADES
KW  -  PROTEIN
KW  -  MEMBRANE
KW  -  TRANSLOCATION
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
EP  - 334
TI  - Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
SN  - 1755-4330
AV  - public
ER  -