eprintid: 10189515
rev_number: 7
eprint_status: archive
userid: 699
dir: disk0/10/18/95/15
datestamp: 2024-03-22 14:40:54
lastmod: 2024-03-22 14:40:54
status_changed: 2024-03-22 14:40:54
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Hailes, Helen C
creators_name: Wang, Yu
creators_name: Li, Yiwen
creators_name: Ni, Yeke
creators_name: Bucar, Dejan-Kresimir
creators_name: Dalby, Paul A
creators_name: Ward, John M
creators_name: Jeffries, Jack
title: A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
ispublished: inpress
divisions: UCL
divisions: B04
divisions: C05
divisions: C06
divisions: F47
divisions: F56
note: © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).
abstract: Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines.
date: 2024-03-01
date_type: published
publisher: Royal Society of Chemistry (RSC)
official_url: http://dx.doi.org/10.1039/d3cy01370g
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2255477
doi: 10.1039/d3cy01370g
lyricists_name: Li, Yiwen
lyricists_name: Bucar, Dejan-Kresimir
lyricists_name: Hailes, Helen
lyricists_name: Ward, John
lyricists_id: YLISX68
lyricists_id: DBUCA58
lyricists_id: HCHAI24
lyricists_id: JMWAR05
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Catalysis Science & Technology
citation:        Hailes, Helen C;    Wang, Yu;    Li, Yiwen;    Ni, Yeke;    Bucar, Dejan-Kresimir;    Dalby, Paul A;    Ward, John M;           Hailes, Helen C;  Wang, Yu;  Li, Yiwen;  Ni, Yeke;  Bucar, Dejan-Kresimir;  Dalby, Paul A;  Ward, John M;  Jeffries, Jack;   - view fewer <#>    (2024)    A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines.                   Catalysis Science & Technology        10.1039/d3cy01370g <https://doi.org/10.1039/d3cy01370g>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10189515/1/d3cy01370g.pdf