eprintid: 10189515 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/18/95/15 datestamp: 2024-03-22 14:40:54 lastmod: 2024-03-22 14:40:54 status_changed: 2024-03-22 14:40:54 type: article metadata_visibility: show sword_depositor: 699 creators_name: Hailes, Helen C creators_name: Wang, Yu creators_name: Li, Yiwen creators_name: Ni, Yeke creators_name: Bucar, Dejan-Kresimir creators_name: Dalby, Paul A creators_name: Ward, John M creators_name: Jeffries, Jack title: A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines ispublished: inpress divisions: UCL divisions: B04 divisions: C05 divisions: C06 divisions: F47 divisions: F56 note: © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). abstract: Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines. date: 2024-03-01 date_type: published publisher: Royal Society of Chemistry (RSC) official_url: http://dx.doi.org/10.1039/d3cy01370g oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2255477 doi: 10.1039/d3cy01370g lyricists_name: Li, Yiwen lyricists_name: Bucar, Dejan-Kresimir lyricists_name: Hailes, Helen lyricists_name: Ward, John lyricists_id: YLISX68 lyricists_id: DBUCA58 lyricists_id: HCHAI24 lyricists_id: JMWAR05 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner full_text_status: public publication: Catalysis Science & Technology citation: Hailes, Helen C; Wang, Yu; Li, Yiwen; Ni, Yeke; Bucar, Dejan-Kresimir; Dalby, Paul A; Ward, John M; Hailes, Helen C; Wang, Yu; Li, Yiwen; Ni, Yeke; Bucar, Dejan-Kresimir; Dalby, Paul A; Ward, John M; Jeffries, Jack; - view fewer <#> (2024) A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines. Catalysis Science & Technology 10.1039/d3cy01370g <https://doi.org/10.1039/d3cy01370g>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10189515/1/d3cy01370g.pdf