TY  - INPR
N2  - Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines.
ID  - discovery10189515
UR  - http://dx.doi.org/10.1039/d3cy01370g
TI  - A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
Y1  - 2024/03/01/
AV  - public
PB  - Royal Society of Chemistry (RSC)
A1  - Hailes, Helen C
A1  - Wang, Yu
A1  - Li, Yiwen
A1  - Ni, Yeke
A1  - Bucar, Dejan-Kresimir
A1  - Dalby, Paul A
A1  - Ward, John M
A1  - Jeffries, Jack
JF  - Catalysis Science & Technology
N1  - © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).
ER  -