TY - INPR N2 - Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines. ID - discovery10189515 UR - http://dx.doi.org/10.1039/d3cy01370g TI - A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines Y1 - 2024/03/01/ AV - public PB - Royal Society of Chemistry (RSC) A1 - Hailes, Helen C A1 - Wang, Yu A1 - Li, Yiwen A1 - Ni, Yeke A1 - Bucar, Dejan-Kresimir A1 - Dalby, Paul A A1 - Ward, John M A1 - Jeffries, Jack JF - Catalysis Science & Technology N1 - © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). ER -