%0 Journal Article
%A Hailes, Helen C
%A Wang, Yu
%A Li, Yiwen
%A Ni, Yeke
%A Bucar, Dejan-Kresimir
%A Dalby, Paul A
%A Ward, John M
%A Jeffries, Jack
%D 2024
%F discovery:10189515
%I Royal Society of Chemistry (RSC)
%J Catalysis Science & Technology
%T A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
%U https://discovery.ucl.ac.uk/id/eprint/10189515/
%X Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines.
%Z © The Royal Society of Chemistry 2024. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).