eprintid: 10189357 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/18/93/57 datestamp: 2024-03-19 13:54:45 lastmod: 2024-03-19 13:54:45 status_changed: 2024-03-19 13:54:45 type: article metadata_visibility: show sword_depositor: 699 creators_name: Han, Kunyuan creators_name: Chen, Jishizhan creators_name: Han, Qinglin creators_name: Sun, Lei creators_name: Dong, Xieping creators_name: Shi, Gengqiang creators_name: Yang, Runhuai creators_name: Wei, Wenqing creators_name: Cheng, Yunzhang title: Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: F45 keywords: Bioantimicrobial materials, nanobactericidal hydrogel microspheres, nanoclays, sandwich-like structures, tissue infections note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Addressing complex tissue infections remains a challenging task because of the lack of effective means, and the limitations of traditional bioantimicrobial materials in single-application scenarios hinder their utility for complex infection sites. Hence, the development of a bioantimicrobial material with broad applicability and potent bactericidal activity is necessary to treat such infections. In this study, a layered lithium magnesium silicate nanoclay (LMS) is used to construct a nanobactericidal platform. This platform exhibits a sandwich-like structure, which is achieved through copper ion modification using a dopamine-mediated metallophenolic network. Moreover, the nanoclay is encapsulated within gelatin methacryloyl (GelMA) hydrogel microspheres for the treatment of complex tissue infections. The results demonstrate that the sandwich-like micro- and nanobactericidal hydrogel microspheres effectively eradicated Staphylococcus aureus (S. aureus) while exhibiting excellent biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Furthermore, the hydrogel microspheres upregulated the expression levels of osteogenic differentiation and angiogenesis-related genes in these cells. In vivo experiments validated the efficacy of sandwich-like micro- and nanobactericidal hydrogel microspheres when injected into deep infected tissues, effectively eliminating bacteria and promoting robust vascular regeneration and tissue repair. Therefore, these innovative sandwich-like micro- and nanobacteriostatic hydrogel microspheres show great potential for treating complex tissue infections. date: 2024-03-11 date_type: published publisher: Wiley-VCH Verlag official_url: https://doi.org/10.1002/mabi.202400027 full_text_type: other language: eng verified: verified_manual elements_id: 2256697 doi: 10.1002/mabi.202400027 medium: Print-Electronic lyricists_name: Chen, Jishizhan lyricists_id: JCHEI07 actors_name: Zahnhausen-Stuber, Petra actors_id: PMZAH20 actors_role: owner full_text_status: restricted publication: Macromolecular Bioscience event_location: Germany issn: 1616-5187 citation: Han, Kunyuan; Chen, Jishizhan; Han, Qinglin; Sun, Lei; Dong, Xieping; Shi, Gengqiang; Yang, Runhuai; ... Cheng, Yunzhang; + view all <#> Han, Kunyuan; Chen, Jishizhan; Han, Qinglin; Sun, Lei; Dong, Xieping; Shi, Gengqiang; Yang, Runhuai; Wei, Wenqing; Cheng, Yunzhang; - view fewer <#> (2024) Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment. Macromolecular Bioscience 10.1002/mabi.202400027 <https://doi.org/10.1002/mabi.202400027>. document_url: https://discovery.ucl.ac.uk/id/eprint/10189357/1/Chen_Nanoclay%20Hydrogel%20Microspheres%20with%20a%20Sandwich-Like%20Structure%20for%20Complex%20Tissue%20Infection%20Treatment.pdf