TY  - JOUR
JF  - Macromolecular Bioscience
PB  - Wiley-VCH Verlag
Y1  - 2024/03/11/
N1  - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions.
ID  - discovery10189357
TI  - Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment
AV  - restricted
UR  - https://doi.org/10.1002/mabi.202400027
A1  - Han, Kunyuan
A1  - Chen, Jishizhan
A1  - Han, Qinglin
A1  - Sun, Lei
A1  - Dong, Xieping
A1  - Shi, Gengqiang
A1  - Yang, Runhuai
A1  - Wei, Wenqing
A1  - Cheng, Yunzhang
SN  - 1616-5187
N2  - Addressing complex tissue infections remains a challenging task because of the lack of effective means, and the limitations of traditional bioantimicrobial materials in single-application scenarios hinder their utility for complex infection sites. Hence, the development of a bioantimicrobial material with broad applicability and potent bactericidal activity is necessary to treat such infections. In this study, a layered lithium magnesium silicate nanoclay (LMS) is used to construct a nanobactericidal platform. This platform exhibits a sandwich-like structure, which is achieved through copper ion modification using a dopamine-mediated metallophenolic network. Moreover, the nanoclay is encapsulated within gelatin methacryloyl (GelMA) hydrogel microspheres for the treatment of complex tissue infections. The results demonstrate that the sandwich-like micro- and nanobactericidal hydrogel microspheres effectively eradicated Staphylococcus aureus (S. aureus) while exhibiting excellent biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Furthermore, the hydrogel microspheres upregulated the expression levels of osteogenic differentiation and angiogenesis-related genes in these cells. In vivo experiments validated the efficacy of sandwich-like micro- and nanobactericidal hydrogel microspheres when injected into deep infected tissues, effectively eliminating bacteria and promoting robust vascular regeneration and tissue repair. Therefore, these innovative sandwich-like micro- and nanobacteriostatic hydrogel microspheres show great potential for treating complex tissue infections.
KW  - Bioantimicrobial materials
KW  -  nanobactericidal hydrogel microspheres
KW  -  nanoclays
KW  -  sandwich-like structures
KW  -  tissue infections
ER  -