TY - JOUR JF - Macromolecular Bioscience PB - Wiley-VCH Verlag Y1 - 2024/03/11/ N1 - This version is the author accepted manuscript. For information on re-use, please refer to the publisher?s terms and conditions. ID - discovery10189357 TI - Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment AV - restricted UR - https://doi.org/10.1002/mabi.202400027 A1 - Han, Kunyuan A1 - Chen, Jishizhan A1 - Han, Qinglin A1 - Sun, Lei A1 - Dong, Xieping A1 - Shi, Gengqiang A1 - Yang, Runhuai A1 - Wei, Wenqing A1 - Cheng, Yunzhang SN - 1616-5187 N2 - Addressing complex tissue infections remains a challenging task because of the lack of effective means, and the limitations of traditional bioantimicrobial materials in single-application scenarios hinder their utility for complex infection sites. Hence, the development of a bioantimicrobial material with broad applicability and potent bactericidal activity is necessary to treat such infections. In this study, a layered lithium magnesium silicate nanoclay (LMS) is used to construct a nanobactericidal platform. This platform exhibits a sandwich-like structure, which is achieved through copper ion modification using a dopamine-mediated metallophenolic network. Moreover, the nanoclay is encapsulated within gelatin methacryloyl (GelMA) hydrogel microspheres for the treatment of complex tissue infections. The results demonstrate that the sandwich-like micro- and nanobactericidal hydrogel microspheres effectively eradicated Staphylococcus aureus (S. aureus) while exhibiting excellent biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Furthermore, the hydrogel microspheres upregulated the expression levels of osteogenic differentiation and angiogenesis-related genes in these cells. In vivo experiments validated the efficacy of sandwich-like micro- and nanobactericidal hydrogel microspheres when injected into deep infected tissues, effectively eliminating bacteria and promoting robust vascular regeneration and tissue repair. Therefore, these innovative sandwich-like micro- and nanobacteriostatic hydrogel microspheres show great potential for treating complex tissue infections. KW - Bioantimicrobial materials KW - nanobactericidal hydrogel microspheres KW - nanoclays KW - sandwich-like structures KW - tissue infections ER -