%0 Journal Article %@ 1616-5187 %A Han, Kunyuan %A Chen, Jishizhan %A Han, Qinglin %A Sun, Lei %A Dong, Xieping %A Shi, Gengqiang %A Yang, Runhuai %A Wei, Wenqing %A Cheng, Yunzhang %D 2024 %F discovery:10189357 %I Wiley-VCH Verlag %J Macromolecular Bioscience %K Bioantimicrobial materials, nanobactericidal hydrogel microspheres, nanoclays, sandwich-like structures, tissue infections %T Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment %U https://discovery.ucl.ac.uk/id/eprint/10189357/ %X Addressing complex tissue infections remains a challenging task because of the lack of effective means, and the limitations of traditional bioantimicrobial materials in single-application scenarios hinder their utility for complex infection sites. Hence, the development of a bioantimicrobial material with broad applicability and potent bactericidal activity is necessary to treat such infections. In this study, a layered lithium magnesium silicate nanoclay (LMS) is used to construct a nanobactericidal platform. This platform exhibits a sandwich-like structure, which is achieved through copper ion modification using a dopamine-mediated metallophenolic network. Moreover, the nanoclay is encapsulated within gelatin methacryloyl (GelMA) hydrogel microspheres for the treatment of complex tissue infections. The results demonstrate that the sandwich-like micro- and nanobactericidal hydrogel microspheres effectively eradicated Staphylococcus aureus (S. aureus) while exhibiting excellent biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Furthermore, the hydrogel microspheres upregulated the expression levels of osteogenic differentiation and angiogenesis-related genes in these cells. In vivo experiments validated the efficacy of sandwich-like micro- and nanobactericidal hydrogel microspheres when injected into deep infected tissues, effectively eliminating bacteria and promoting robust vascular regeneration and tissue repair. Therefore, these innovative sandwich-like micro- and nanobacteriostatic hydrogel microspheres show great potential for treating complex tissue infections. %Z This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.