@article{discovery10189357, note = {This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.}, publisher = {Wiley-VCH Verlag}, month = {March}, year = {2024}, title = {Nanoclay Hydrogel Microspheres with a Sandwich-Like Structure for Complex Tissue Infection Treatment}, journal = {Macromolecular Bioscience}, keywords = {Bioantimicrobial materials, nanobactericidal hydrogel microspheres, nanoclays, sandwich-like structures, tissue infections}, abstract = {Addressing complex tissue infections remains a challenging task because of the lack of effective means, and the limitations of traditional bioantimicrobial materials in single-application scenarios hinder their utility for complex infection sites. Hence, the development of a bioantimicrobial material with broad applicability and potent bactericidal activity is necessary to treat such infections. In this study, a layered lithium magnesium silicate nanoclay (LMS) is used to construct a nanobactericidal platform. This platform exhibits a sandwich-like structure, which is achieved through copper ion modification using a dopamine-mediated metallophenolic network. Moreover, the nanoclay is encapsulated within gelatin methacryloyl (GelMA) hydrogel microspheres for the treatment of complex tissue infections. The results demonstrate that the sandwich-like micro- and nanobactericidal hydrogel microspheres effectively eradicated Staphylococcus aureus (S. aureus) while exhibiting excellent biocompatibility with bone marrow-derived mesenchymal stem cells (BMSCs) and human umbilical vein endothelial cells (HUVECs). Furthermore, the hydrogel microspheres upregulated the expression levels of osteogenic differentiation and angiogenesis-related genes in these cells. In vivo experiments validated the efficacy of sandwich-like micro- and nanobactericidal hydrogel microspheres when injected into deep infected tissues, effectively eliminating bacteria and promoting robust vascular regeneration and tissue repair. Therefore, these innovative sandwich-like micro- and nanobacteriostatic hydrogel microspheres show great potential for treating complex tissue infections.}, url = {https://doi.org/10.1002/mabi.202400027}, issn = {1616-5187}, author = {Han, Kunyuan and Chen, Jishizhan and Han, Qinglin and Sun, Lei and Dong, Xieping and Shi, Gengqiang and Yang, Runhuai and Wei, Wenqing and Cheng, Yunzhang} }