eprintid: 10188471
rev_number: 6
eprint_status: archive
userid: 699
dir: disk0/10/18/84/71
datestamp: 2024-03-06 12:48:21
lastmod: 2024-03-06 12:48:21
status_changed: 2024-03-06 12:48:21
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Koller, Garrit
creators_name: Foschi, Federico
creators_name: Mitchell, Philip
creators_name: Witherden, Elizabeth
creators_name: Bruce, Kenneth
creators_name: Mannocci, Francesco
title: Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence
ispublished: pub
divisions: UCL
divisions: B02
divisions: C10
divisions: D21
divisions: H09
keywords: amoeba-resistant bacteria, bacterial fitness, endodontics, infection, microbial ecology, microbiology, polymicrobial infection
note: © 2020 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
abstract: BACKGROUND: Infections of the root canal space involve polymicrobial biofilms and lead to chronic, low grade inflammatory responses arising from the seeding of microbes and by-products. Acute exacerbation and/or disseminating infections occur when established microbial communities undergo sudden changes in phenotypic behaviour. METHODS: Within clinical endodontic infections, we assessedcategorical determinants comprising, and changing microbial composition of, chronic polymicrobial infections and their association with amoebae. After standardised assessment, primary or secondary infections underwent sampling and DNA processing, targeting bacteria, fungi and amoebae, including 16S high-throughput sequencing. After taxonomic assignment, community composition was correlated with clinical signs and symptoms. Diversity and abundance analyses were carried out in relation to the presence of non-bacterial amplicons. RESULTS: Clinical specimens revealed two distinct community clusters, where specific changes correlated with clinical signs. An association between the compositions of microbiomes was found between these groups and the presence of Entamoeba gingivalis in 44% of cases. When amoebae were present in endodontic infections, we demonstrate changes in microbial community structure that mirror those observed in treatment-resistant or recurrent infections. CONCLUSIONS: Amoeba are present in endodontic infections at a high prevalence, and may promote increased virulence by enrichment for phagocytosis-resistant bacteria.
date: 2020
date_type: published
publisher: MDPI AG
official_url: http://dx.doi.org/10.3390/jcm9113700
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2254046
doi: 10.3390/jcm9113700
medium: Electronic
pii: jcm9113700
lyricists_name: Foschi, Federico
lyricists_id: FFOSC10
actors_name: Foschi, Federico
actors_id: FFOSC10
actors_role: owner
full_text_status: public
publication: Journal of Clinical Medicine Status:
volume: 9
number: 11
article_number: E3700
event_location: Switzerland
citation:        Koller, Garrit;    Foschi, Federico;    Mitchell, Philip;    Witherden, Elizabeth;    Bruce, Kenneth;    Mannocci, Francesco;      (2020)    Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence.                   Journal of Clinical Medicine Status: , 9  (11)    , Article E3700.  10.3390/jcm9113700 <https://doi.org/10.3390/jcm9113700>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10188471/1/Amoebae%20in%20Chronic%2C%20Polymicrobial%20Endodontic%20Infections%20Are%20Associated%20with%20Altered%20Microbial%20Communities%20of%20Increased%20Virul.pdf