eprintid: 10188097
rev_number: 9
eprint_status: archive
userid: 699
dir: disk0/10/18/80/97
datestamp: 2024-02-28 15:02:37
lastmod: 2024-02-28 15:02:37
status_changed: 2024-02-28 15:02:37
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Poulton, Beth C
creators_name: Colman, Fraser
creators_name: Anthousi, Amalia
creators_name: Sattelle, David B
creators_name: Lycett, Gareth J
title: Aedes aegypti CCEae3A carboxylase expression confers carbamate, organophosphate and limited pyrethroid resistance in a model transgenic mosquito
ispublished: inpress
divisions: UCL
divisions: B02
divisions: C10
divisions: D17
divisions: K71
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abstract: Insecticide resistance is a serious threat to our ability to control mosquito vectors which transmit pathogens including malaria parasites and arboviruses. Understanding the underlying mechanisms is an essential first step in tackling the challenges presented by resistance. This study aimed to functionally characterise the carboxylesterase, CCEae3A, the elevated expression of which has been implicated in temephos resistance in Aedes aegypti and Aedes albopictus larvae. Using our GAL4/UAS expression system, already established in insecticide-sensitive Anopheles gambiae mosquitoes, we produced transgenic An. gambiae mosquitoes that express an Ae. aegypti CCEae3A ubiquitously. This new transgenic line permits examination of CCEae3A expression in a background in which there is not a clear orthologue in Vectorbase and allows comparison with existing An. gambiae GAL4-UAS lines. Insecticide resistance profiling of these transgenic An. gambiae larvae indicated significant increases in resistance ratio for three organophosphate insecticides, temephos (6), chloropyriphos (6.6) and fenthion (3.2) when compared to the parental strain. Cross resistance to adulticides from three major insecticide classes: organophosphates (malathion, fenitrothion and pirimiphos methyl), carbamates (bendiocarb and propoxur) and pyrethroid (alpha-cypermethrin) was also detected. Resistance to certain organophosphates and carbamates validates conclusions drawn from previous expression and phenotypic data. However, detection of resistance to pirimiphos methyl and alphacypermethrin has not previously been formally associated with CCEae3A, despite occurring in Ae. aegypti strains where this gene was upregulated. Our findings highlight the importance of characterising individual resistance mechanisms, thereby ensuring accurate information is used to guide future vector control strategies.
date: 2024-02-20
date_type: published
publisher: Public Library of Science (PLoS)
official_url: http://dx.doi.org/10.1371/journal.pntd.0011595
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2248764
doi: 10.1371/journal.pntd.0011595
medium: Print-Electronic
pii: PNTD-D-23-01041
lyricists_name: Sattelle, David
lyricists_id: DBSAT61
actors_name: Sattelle, David
actors_id: DBSAT61
actors_role: owner
full_text_status: public
publication: PLOS Neglected Tropical Diseases
volume: 18
number: 2
article_number: e0011595
event_location: United States
editors_name: Acosta-Serrano, Álvaro
citation:        Poulton, Beth C;    Colman, Fraser;    Anthousi, Amalia;    Sattelle, David B;    Lycett, Gareth J;      (2024)    Aedes aegypti CCEae3A carboxylase expression confers carbamate, organophosphate and limited pyrethroid resistance in a model transgenic mosquito.                   PLOS Neglected Tropical Diseases , 18  (2)    , Article e0011595.  10.1371/journal.pntd.0011595 <https://doi.org/10.1371/journal.pntd.0011595>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10188097/1/Sattelle_Poulton%20et%20al%202023%20-%20Revised%20manuscript%20%28clean%20copy_figs%29.pdf