TY  - JOUR
IS  - 20
VL  - 10
PB  - BIO-PROTOCOL
JF  - bio-protocol
TI  - Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter
A1  - Swire, Matthew
A1  - Ffrench-Constant, Charles
Y1  - 2020/10/20/
UR  - http://dx.doi.org/10.21769/bioprotoc.3792
N1  - © The Authors 2023. Swire and ffrench-Constant. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/).
EP  - 9
ID  - discovery10187828
N2  - Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse grey matter. This protocol enables the investigation of myelination without the need for reporter mice or technically challenging protocols, aiding the investigation of factors influencing myelin production in the brain.
AV  - public
KW  - Oligodendrocyte
KW  -  Myelin
KW  -  Myelination
KW  -  CNS
KW  -  Immunofluorescence
ER  -