TY - JOUR IS - 20 VL - 10 PB - BIO-PROTOCOL JF - bio-protocol TI - Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter A1 - Swire, Matthew A1 - Ffrench-Constant, Charles Y1 - 2020/10/20/ UR - http://dx.doi.org/10.21769/bioprotoc.3792 N1 - © The Authors 2023. Swire and ffrench-Constant. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). EP - 9 ID - discovery10187828 N2 - Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse grey matter. This protocol enables the investigation of myelination without the need for reporter mice or technically challenging protocols, aiding the investigation of factors influencing myelin production in the brain. AV - public KW - Oligodendrocyte KW - Myelin KW - Myelination KW - CNS KW - Immunofluorescence ER -