eprintid: 10185912 rev_number: 9 eprint_status: archive userid: 699 dir: disk0/10/18/59/12 datestamp: 2024-01-22 08:39:22 lastmod: 2024-01-22 08:39:22 status_changed: 2024-01-22 08:39:22 type: article metadata_visibility: show sword_depositor: 699 creators_name: He, Chloe creators_name: Karpavičiūtė, Neringa creators_name: Hariharan, Rishabh creators_name: Lees, Lilly creators_name: Jacques, Céline creators_name: Ferrand, Timothy creators_name: Chambost, Jérôme creators_name: Wouters, Koen creators_name: Malmsten, Jonas creators_name: Miller, Ryan creators_name: Zaninovic, Nikica creators_name: Vasconcelos, Francisco creators_name: Hickman, Cristina title: Seeking arrangements: cell contact as a cleavage-stage biomarker ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: F48 keywords: 3D reconstruction; Cleavage stage; Embryology; Cell arrangement; Cell contact; Network analysis note: Copyright © 2023 The Authors. Published by Elsevier Ltd on behalf of Reproductive Healthcare Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). abstract: Research question: What can three-dimensional cell contact networks tell us about the developmental potential of cleavage-stage human embryos? // Design: This pilot study was a retrospective analysis of two Embryoscope imaging datasets from two clinics. An artificial intelligence system was used to reconstruct the three-dimensional structure of embryos from 11-plane focal stacks. Networks of cell contacts were extracted from the resulting embryo three-dimensional models and each embryo's mean contacts per cell was computed. Unpaired t-tests and receiver operating characteristic curve analysis were used to statistically analyse mean cell contact outcomes. Cell contact networks from different embryos were compared with identical embryos with similar cell arrangements. // Results: At t4, a higher mean number of contacts per cell was associated with greater rates of blastulation and blastocyst quality. No associations were found with biochemical pregnancy, live birth, miscarriage or ploidy. At t8, a higher mean number of contacts was associated with increased blastocyst quality, biochemical pregnancy and live birth. No associations were found with miscarriage or aneuploidy. Mean contacts at t4 weakly correlated with those at t8. Four-cell embryos fell into nine distinct cell arrangements; the five most common accounted for 97% of embryos. Eight-cell embryos, however, displayed a greater degree of variation with 59 distinct cell arrangements. // Conclusions: Evidence is provided for the clinical relevance of cleavage-stage cell arrangement in the human preimplantation embryo beyond the four-cell stage, which may improve selection techniques for day-3 transfers. This pilot study provides a strong case for further investigation into spatial biomarkers and three-dimensional morphokinetics. date: 2024-03 date_type: published publisher: Elsevier BV official_url: http://dx.doi.org/10.1016/j.rbmo.2023.103654 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2105679 doi: 10.1016/j.rbmo.2023.103654 lyricists_name: He, Chloe lyricists_id: PHEXX73 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner full_text_status: public publication: Reproductive BioMedicine Online volume: 48 number: 3 article_number: 103654 issn: 1472-6483 citation: He, Chloe; Karpavičiūtė, Neringa; Hariharan, Rishabh; Lees, Lilly; Jacques, Céline; Ferrand, Timothy; Chambost, Jérôme; ... Hickman, Cristina; + view all <#> He, Chloe; Karpavičiūtė, Neringa; Hariharan, Rishabh; Lees, Lilly; Jacques, Céline; Ferrand, Timothy; Chambost, Jérôme; Wouters, Koen; Malmsten, Jonas; Miller, Ryan; Zaninovic, Nikica; Vasconcelos, Francisco; Hickman, Cristina; - view fewer <#> (2024) Seeking arrangements: cell contact as a cleavage-stage biomarker. Reproductive BioMedicine Online , 48 (3) , Article 103654. 10.1016/j.rbmo.2023.103654 <https://doi.org/10.1016/j.rbmo.2023.103654>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10185912/1/He_1-s2.0-S1472648323007538.-main.pdf