eprintid: 10185145
rev_number: 11
eprint_status: archive
userid: 699
dir: disk0/10/18/51/45
datestamp: 2024-01-09 10:46:43
lastmod: 2024-01-09 10:46:43
status_changed: 2024-01-09 10:46:43
type: conference_item
metadata_visibility: show
sword_depositor: 699
creators_name: Seo, Jegak
creators_name: Kloprogge, Frank
creators_name: Smith, Andrew
creators_name: Ciric, Lena
title: O02 Impact of dietary habits on AMR development in human gut bacteria: insights from a hollow fibre infection model
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F44
note: Copyright © The Author(s) 2024. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
abstract: Background:
As efforts to reduce antibiotic exposure through medical prescriptions have made significant progress, the potential consequences of residual antibiotics in animal-based food sources have been somewhat overlooked. The question of whether chronic exposure to antibiotic remnants in human diets could lead to the development of antimicrobial resistance (AMR) in human gut bacteria remains largely unanswered. This research aimed to address this gap by utilizing the hollow fibre infection model (HFIM) to investigate whether routine consumption of antibiotics from an omnivorous diet contributes to the emergence of resistance in a previously antibiotic-susceptible strain of E. coli isolated from the human gut.
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Methods:
To select the antibiotic mixtures for our study, we conducted a comprehensive diet survey involving 131 participants. Additionally, we employed LC-MS to quantify antibiotic concentrations in the most commonly consumed animal-based food products. We rigorously analysed triplicates of 34 food and beverage items, encompassing beef, chicken, pork, fish, and dairy, to detect and measure the concentrations of 10 specific antibiotics. Our findings revealed the presence of low levels of nine antibiotics across the samples, with amoxicillin and trimethoprim being the most frequently detected antibiotics. Alarmingly, 12 out of 34 products exceeded the acceptable daily intake levels for amoxicillin, ampicillin, and enrofloxacin. We further adapted the estimated daily intake formula to calculate antibiotic combinations and their intake from each meal. Subsequently, we subjected bacterial cells to the HFIM for 7 days, exposing them to three antibiotic ‘meals’ daily. We collected samples from the HFIM every 24 h to monitor changes in resistance phenotypes through MIC testing, as well as for subsequent transcriptomic analysis.
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Results:
Astonishingly, we observed the development of resistance to both individual antibiotics and combinations of antibiotics from the very first day, with resistance levels progressively increasing over the course of the week. Interestingly, the rate at which MICs increased was most rapid in the initial days, followed by a slower rate of increase while MICs continued to rise. This study provides compelling evidence that chronic exposure to low levels of antibiotics, akin to those found in animal-derived foods, can lead to rapid resistance development across multiple antibiotics within human gut bacteria. Further examination of samples from the HFIM through RT-PCR and WGS is warranted to elucidate the mechanisms underlying resistance development in the gut environment due to dietary factors.
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Conclusions:
Our research highlights the urgent need for greater scrutiny of antibiotic residues in the food chain and emphasizes the potential role of dietary habits in driving AMR development within human gut bacteria. Understanding the underlying mechanisms is crucial for devising effective strategies to mitigate this emerging public health concern.
date: 2024-01-03
date_type: published
publisher: Oxford University Press (OUP)
official_url: http://dx.doi.org/10.1093/jacamr/dlad143.002
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2137846
doi: 10.1093/jacamr/dlad143.002
lyricists_name: Seo, Jegak
lyricists_id: JSEOX08
actors_name: Seo, Jegak
actors_id: JSEOX08
actors_role: owner
full_text_status: public
pres_type: presentation
publication: JAC-Antimicrobial Resistance
volume: 6
number: Suppleme
event_title: Infection 2023: BSAC Winter Conference
event_location: London, UK
event_dates: 30 November-01 December 2023
issn: 2632-1823
citation:        Seo, Jegak;    Kloprogge, Frank;    Smith, Andrew;    Ciric, Lena;      (2024)    O02 Impact of dietary habits on AMR development in human gut bacteria: insights from a hollow fibre infection model.                   Presented at: Infection 2023: BSAC Winter Conference, London, UK.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10185145/1/dlad143.002.pdf