<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> .
<> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10180184> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/title> "Large-scale manufacturing of base-edited chimeric antigen receptor T cells"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/abstract> "Base editing is a revolutionary gene-editing technique enabling the introduction of point mutations into the genome without generating detrimental DNA double-stranded breaks. Base-editing enzymes are commonly delivered in the form of modified linear messenger RNA (mRNA) that is costly to produce. Here, we address this problem by developing a simple protocol for manufacturing base-edited cells using circular RNA (circRNA), which is less expensive to synthesize. Compared with linear mRNA, higher editing efficiencies were achieved with circRNA, enabling an 8-fold reduction in the amount of RNA required. We used this protocol to manufacture a clinical dose (1 × 108 cells) of base-edited chimeric antigen receptor (CAR) T cells lacking expression of the inhibitory receptor, PD-1. Editing efficiencies of up to 86% were obtained using 0.25 μg circRNA/1 × 106 cells. Increased editing efficiencies with circRNA were attributed to more efficient translation. These results suggest that circRNA, which is less expensive to produce than linear mRNA, is a viable option for reducing the cost of manufacturing base-edited cells at scale."^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/date> "2023-12-14" .
<https://discovery.ucl.ac.uk/id/document/1670106> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/volume> "31" .
<https://discovery.ucl.ac.uk/id/org/ext-464ff5f0fac4572c5372d3e7fc978be1> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> .
<https://discovery.ucl.ac.uk/id/org/ext-464ff5f0fac4572c5372d3e7fc978be1> <http://xmlns.com/foaf/0.1/name> "Elsevier BV"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/publisher> <https://discovery.ucl.ac.uk/id/org/ext-464ff5f0fac4572c5372d3e7fc978be1> .
<https://discovery.ucl.ac.uk/id/publication/ext-4460f5173f0874420a0a3e87bef5fa75> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> .
<https://discovery.ucl.ac.uk/id/publication/ext-4460f5173f0874420a0a3e87bef5fa75> <http://xmlns.com/foaf/0.1/name> "Molecular Therapy: Methods & Clinical Development"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-4460f5173f0874420a0a3e87bef5fa75> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_2> <https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_7> <https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-9d94316a2638caee03739a27dc585063> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_8> <https://discovery.ucl.ac.uk/id/person/ext-9d94316a2638caee03739a27dc585063> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_9> <https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_10> <https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_11> <https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_12> <https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> .
<https://discovery.ucl.ac.uk/id/eprint/10180184> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10180184#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10180184#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_13> <https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> .
<https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> <http://xmlns.com/foaf/0.1/givenName> "Leila"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> <http://xmlns.com/foaf/0.1/familyName> "Mekkaoui"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-2910bf6742b28f794e6ada02d50b67ee> <http://xmlns.com/foaf/0.1/name> "Leila Mekkaoui"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> <http://xmlns.com/foaf/0.1/givenName> "Jasmine"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> <http://xmlns.com/foaf/0.1/familyName> "Huang"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-970e92b3701343e384576addac05998d> <http://xmlns.com/foaf/0.1/name> "Jasmine Huang"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> <http://xmlns.com/foaf/0.1/givenName> "Katarina"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> <http://xmlns.com/foaf/0.1/familyName> "Lamb"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d0f53a9ea4992ad0c4109f7ce1acd996> <http://xmlns.com/foaf/0.1/name> "Katarina Lamb"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> <http://xmlns.com/foaf/0.1/givenName> "Martin"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> <http://xmlns.com/foaf/0.1/familyName> "Pule"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-028b2b47743656dc0039691087265f21> <http://xmlns.com/foaf/0.1/name> "Martin Pule"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> <http://xmlns.com/foaf/0.1/givenName> "Koki"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> <http://xmlns.com/foaf/0.1/familyName> "Lilova"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-b82e2fa8d857445f982dfd608fd4edde> <http://xmlns.com/foaf/0.1/name> "Koki Lilova"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> <http://xmlns.com/foaf/0.1/givenName> "Rosie"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> <http://xmlns.com/foaf/0.1/familyName> "Woodruff"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-0e7f65deca8928b825d11fed51de2937> <http://xmlns.com/foaf/0.1/name> "Rosie Woodruff"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> <http://xmlns.com/foaf/0.1/givenName> "Katerina"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> <http://xmlns.com/foaf/0.1/familyName> "Smetanova"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-58d65d0eaee183a84488729424336baa> <http://xmlns.com/foaf/0.1/name> "Katerina Smetanova"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> <http://xmlns.com/foaf/0.1/givenName> "Claire"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> <http://xmlns.com/foaf/0.1/familyName> "Roddie"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-11021db6ed4e442b9c650eb8cee53549> <http://xmlns.com/foaf/0.1/name> "Claire Roddie"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> <http://xmlns.com/foaf/0.1/givenName> "James"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> <http://xmlns.com/foaf/0.1/familyName> "Sillibourne"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-12ccf0940342b435b3bd117c8b14e432> <http://xmlns.com/foaf/0.1/name> "James Sillibourne"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> <http://xmlns.com/foaf/0.1/givenName> "Christopher"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> <http://xmlns.com/foaf/0.1/familyName> "Allen"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-22a1e344f2a4a529a421c66187da318d> <http://xmlns.com/foaf/0.1/name> "Christopher Allen"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> <http://xmlns.com/foaf/0.1/givenName> "Gerardo Santiago"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> <http://xmlns.com/foaf/0.1/familyName> "Toledo"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-d8bdc332aeb22e3de9a7ebc3cfb8e46c> <http://xmlns.com/foaf/0.1/name> "Gerardo Santiago Toledo"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> <http://xmlns.com/foaf/0.1/givenName> "Farhaan"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> <http://xmlns.com/foaf/0.1/familyName> "Parekh"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-7dff00190f8a12aa704a3b3e5ff191f4> <http://xmlns.com/foaf/0.1/name> "Farhaan Parekh"^^<http://www.w3.org/2001/XMLSchema#string> .
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