TY - INPR N2 - 1. Above-ground biomass (AGB) is an important metric used to quantify the mass of carbon stored in terrestrial ecosystems. For forests, this is routinely estimated at the plot scale (typically 1?ha) using inventory measurements and allometry. In recent years, terrestrial laser scanning (TLS) has appeared as a disruptive technology that can generate a more accurate assessment of tree and plot scale AGB; however, operationalising TLS methods has had to overcome a number of challenges. One such challenge is the segmentation of individual trees from plot level point clouds that are required to estimate woody volume, this is often done manually (e.g. with interactive point cloud editing software) and can be very time consuming. / 2. Here we present TLS2trees, an automated processing pipeline and set of Python command line tools that aims to redress this processing bottleneck. TLS2trees consists of existing and new methods and is specifically designed to be horizontally scalable. The processing pipeline is demonstrated on 7.5?ha of TLS data captured across 10 plots of seven forest types; from open savanna to dense tropical rainforest. / 3. A total of 10,557 trees are segmented with TLS2trees: these are compared to 1281 manually segmented trees. Results indicate that TLS2trees performs well, particularly for larger trees (i.e. the cohort of largest trees that comprise 50% of total plot volume), where plot-wise tree volume bias is ą0.4?m3 and %RMSE is 60%. Segmentation performance decreases for smaller trees, for example where DBH ?10?cm; a number of reasons are suggested including performance of semantic segmentation step. / 4. The volume and scale of TLS data captured in forest plots is increasing. It is suggested that to fully utilise this data for activities such as monitoring, reporting and verification or as reference data for satellite missions an automated processing pipeline, such as TLS2trees, is required. To facilitate improvements to TLS2trees, as well as modification for other laser scanning modes (e.g. mobile and UAV laser scanning), TLS2trees is a free and open-source software. ID - discovery10179836 UR - https://doi.org/10.1111/2041-210x.14233 TI - TLS2trees: A scalable tree segmentation pipeline for TLS data SN - 2041-210X Y1 - 2023/10/21/ AV - public KW - above-ground biomass KW - Forest KW - FOSS KW - segmentation KW - terrestrial laser scanning PB - Wiley JF - Methods in Ecology and Evolution A1 - Wilkes, Phil A1 - Disney, Mathias A1 - Armston, John A1 - Bartholomeus, Harm A1 - Bentley, Lisa A1 - Brede, Benjamin A1 - Burt, Andrew A1 - Calders, Kim A1 - Chavana?Bryant, Cecilia A1 - Clewley, Daniel A1 - Duncanson, Laura A1 - Forbes, Brieanne A1 - Krisanski, Sean A1 - Malhi, Yadvinder A1 - Moffat, David A1 - Origo, Niall A1 - Shenkin, Alexander A1 - Yang, Wanxin N1 - Copyright Š 2023 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ER -