TY  - JOUR
VL  - 12
UR  - https://doi.org/10.3390/cells12202490
A1  - Moezinia, Carine
A1  - Wong, Valerie
A1  - Watson, James
A1  - Nagib, Lydia
A1  - Lopez Garces, Sandra
A1  - Zhang, Siyu
A1  - Ahmed Abdi, Bahja
A1  - Newton, Florence
A1  - Abraham, David
A1  - Stratton, Richard
AV  - public
N2  - Systemic sclerosis (SSc) is a multisystem connective tissue disease characterised by pathological processes involving autoimmunity, vasculopathy and resultant extensive skin and organ fibrosis. Recent studies have demonstrated activation and aberrant wound healing responses in the epithelial layer of the skin in this disease, implicating the epithelial keratinocytes as a source of pro-fibrotic and inflammatory mediators. In this paper, we investigated the role of Immunoglobulin G (IgG) autoantibodies directed against epithelial cells, as potential initiators and propagators of pathological keratocyte activation and the ensuing SSc fibrotic cascade. A keratinocyte cell-based ELISA is used to evaluate the binding of SSc IgG. SSc skin biopsies were stained by immunofluorescence for the presence of IgG in the keratinocyte layer. Moreover, IgG purified from SSc sera was evaluated for the potential to activate keratinocytes in tissue culture and to induce TLR2 and 3 signalling in reporter cell lines. We demonstrate enhanced binding of SSc IgG to keratinocytes and the activation of these cells leading to the release of IL-1?, representing a potential initiating pathway in this disease.
JF  - Cells
KW  - scleroderma; autoantibody; epithelial cell; fibrosis
ID  - discovery10179789
IS  - 20
PB  - MDPI AG
N1  - © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
TI  - Autoantibodies Which Bind to and Activate Keratinocytes in Systemic Sclerosis
Y1  - 2023/10/20/
SN  - 2073-4409
ER  -