TY - JOUR TI - KIR-HLA interactions extend human CD8+ T cell lifespan in vivo AV - public VL - 133 Y1 - 2023/// IS - 12 N1 - © 2023 Zhang et al. This work is licensed under the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). N2 - BACKGROUND. There is increasing evidence, in transgenic mice and in vitro, that inhibitory killer cell immunoglobulin-like receptors (iKIRs) can modulate T cell responses. Furthermore, we have previously shown that iKIRs are an important determinant of T cell?mediated control of chronic viral infection and that these results are consistent with an increase in the CD8+ T cell lifespan due to iKIR-ligand interactions. Here, we tested this prediction and investigated whether iKIRs affect T cell lifespan in humans in vivo. METHODS. We used stable isotope labeling with deuterated water to quantify memory CD8+ T cell survival in healthy individuals and patients with chronic viral infections. RESULTS. We showed that an individual?s iKIR-ligand genotype was a significant determinant of CD8+ T cell lifespan: in individuals with 2 iKIR-ligand gene pairs, memory CD8+ T cells survived, on average, for 125 days; in individuals with 4 iKIR-ligand gene pairs, the memory CD8+ T cell lifespan doubled to 250 days. Additionally, we showed that this survival advantage was independent of iKIR expression by the T cell of interest and, further, that the iKIR-ligand genotype altered the CD8+ and CD4+ T cell immune aging phenotype. CONCLUSIONS. Together, these data reveal an unexpectedly large effect of iKIR genotype on T cell survival. ID - discovery10174707 PB - American Society for Clinical Investigation UR - https://doi.org/10.1172/JCI169496 JF - Journal of Clinical Investigation A1 - Zhang, Y A1 - Yan, AWC A1 - Boelen, L A1 - Hadcocks, L A1 - Salam, A A1 - Gispert, DP A1 - Spanos, L A1 - Bitria, LM A1 - Nemat-Gorgani, N A1 - Traherne, JA A1 - Roberts, C A1 - Koftori, D A1 - Taylor, GP A1 - Forton, D A1 - Norman, PJ A1 - Marsh, SGE A1 - Busch, R A1 - Macallan, DC A1 - Asquith, B ER -