eprintid: 10165186
rev_number: 6
eprint_status: archive
userid: 699
dir: disk0/10/16/51/86
datestamp: 2023-02-20 11:48:09
lastmod: 2023-02-20 11:48:09
status_changed: 2023-02-20 11:48:09
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Ester, Mar Casajuana
creators_name: Day, Richard M
title: Production and Utility of Extracellular Vesicles with 3D Culture Methods
ispublished: pub
divisions: UCL
divisions: B02
divisions: B04
divisions: C10
divisions: C05
divisions: D17
divisions: F51
divisions: G94
keywords: extracellular vesicles; 3D culture; scaffolds; bioreactors; spheroids
note: © 2023 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
abstract: In recent years, extracellular vesicles (EVs) have emerged as promising biomarkers, cell-free therapeutic agents, and drug delivery carriers. Despite their great clinical potential, poor yield and unscalable production of EVs remain significant challenges. When using 3D culture methods, such as scaffolds and bioreactors, large numbers of cells can be expanded and the cell environment can be manipulated to control the cell phenotype. This has been employed to successfully increase the production of EVs as well as to enhance their therapeutic effects. The physiological relevance of 3D cultures, such as spheroids, has also provided a strategy for understanding the role of EVs in the pathogenesis of several diseases and to evaluate their role as tools to deliver drugs. Additionally, 3D culture methods can encapsulate EVs to achieve more sustained therapeutic effects as well as prevent premature clearance of EVs to enable more localised delivery and concentrated exosome dosage. This review highlights the opportunities and drawbacks of different 3D culture methods and their use in EV research.
date: 2023-02
date_type: published
publisher: MDPI AG
official_url: https://doi.org/10.3390/pharmaceutics15020663
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 2005891
doi: 10.3390/pharmaceutics15020663
lyricists_name: Casajuana Ester, Mar
lyricists_name: Day, Richard
lyricists_id: MCASA91
lyricists_id: RDAYX34
actors_name: Casajuana Ester, Mar
actors_id: MCASA91
actors_role: owner
full_text_status: public
publication: Pharmaceutics
volume: 15
number: 2
pagerange: 663-663
citation:        Ester, Mar Casajuana;    Day, Richard M;      (2023)    Production and Utility of Extracellular Vesicles with 3D Culture Methods.                   Pharmaceutics , 15  (2)   p. 663.    10.3390/pharmaceutics15020663 <https://doi.org/10.3390/pharmaceutics15020663>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10165186/1/pharmaceutics-15-00663-v2.pdf