eprintid: 10164920 rev_number: 8 eprint_status: archive userid: 699 dir: disk0/10/16/49/20 datestamp: 2023-02-15 14:28:43 lastmod: 2024-11-08 16:25:13 status_changed: 2023-02-15 14:28:43 type: article metadata_visibility: show sword_depositor: 699 creators_name: Privitera, Laura creators_name: Musleh, Layla creators_name: Paraboschi, Irene creators_name: Ogunlade, Olumide creators_name: Ogunbiyi, Olumide creators_name: Hutchinson, J Ciaran creators_name: Sebire, Neil creators_name: Beard, Paul creators_name: Giuliani, Stefano title: Dynamic Changes in Microvascular Density Can Predict Viable and Non-Viable Areas in High-Risk Neuroblastoma ispublished: pub subjects: GOSH divisions: UCL divisions: B02 divisions: B04 divisions: C05 divisions: D13 divisions: F42 divisions: G22 divisions: G25 keywords: high-risk neuroblastoma, image-guided surgery, microvascular density, optical imaging, photoacoustic imaging note: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). abstract: Despite aggressive treatments, the prognosis of high-risk NB remains poor. Surgical oncology needs innovative intraoperative devices to help surgeons discriminate malignant tissue from necrotic and surrounding healthy tissues. Changes within the tumor vasculature could be used intraoperatively as a diagnostic tool to guide surgical resection. Here, we retrospectively analyzed the mean vascular density (MVD) of different NB subtypes at diagnosis and after induction chemotherapy using scanned histological samples. One patient was prospectively enrolled, and an ex vivo photoacoustic imaging (PAI) scan was performed on two representative sections to assess its capacity to discriminate different tumor regions. We found that post-chemotherapy, viable areas of differentiating NBs and ganglioneuroblastomas are associated with higher MVD compared to poorly differentiated NBs. Early necrotic regions showed higher MVD than late necrotic and viable regions. Finally, calcified areas showed significantly lower MVD than any other histological component. The acquired PAI images showed a good high-resolution ex vivo 3D delineation of NB margins. Overall, these results suggest that a high-definition preclinical imaging device such as PAI could potentially be exploited to guide surgical resection by identifying different vasculature signatures. date: 2023-02-01 date_type: published publisher: MDPI AG official_url: https://doi.org/10.3390/cancers15030917 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 2005149 doi: 10.3390/cancers15030917 medium: Electronic pii: cancers15030917 lyricists_name: Privitera, Laura lyricists_name: Sebire, Neil lyricists_name: Ogunlade, Olumide lyricists_name: Giuliani, Stefano lyricists_id: LPRIV88 lyricists_id: NJSEB45 lyricists_id: OOOGU48 lyricists_id: SGIUL32 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner funding_acknowledgements: MR/T005491/1 [Medical Research Council]; 222111/Z/20/Z [Wellcome Trust] full_text_status: public publication: Cancers volume: 15 number: 3 article_number: 917 event_location: Switzerland citation: Privitera, Laura; Musleh, Layla; Paraboschi, Irene; Ogunlade, Olumide; Ogunbiyi, Olumide; Hutchinson, J Ciaran; Sebire, Neil; ... Giuliani, Stefano; + view all <#> Privitera, Laura; Musleh, Layla; Paraboschi, Irene; Ogunlade, Olumide; Ogunbiyi, Olumide; Hutchinson, J Ciaran; Sebire, Neil; Beard, Paul; Giuliani, Stefano; - view fewer <#> (2023) Dynamic Changes in Microvascular Density Can Predict Viable and Non-Viable Areas in High-Risk Neuroblastoma. Cancers , 15 (3) , Article 917. 10.3390/cancers15030917 <https://doi.org/10.3390/cancers15030917>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10164920/1/cancers-15-00917.pdf