eprintid: 10162404 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/16/24/04 datestamp: 2023-01-04 10:02:22 lastmod: 2023-01-04 10:02:22 status_changed: 2023-01-04 10:02:22 type: article metadata_visibility: show sword_depositor: 699 creators_name: Hernández-Bejarano, M creators_name: Gestri, G creators_name: Monfries, C creators_name: Tucker, L creators_name: Dragomir, EI creators_name: Bianco, IH creators_name: Bovolenta, P creators_name: Wilson, SW creators_name: Cavodeassi, F title: Foxd1-dependent induction of a temporal retinal character is required for visual function ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: F96 divisions: G02 keywords: Foxd1, Rx3, Retinal patterning, Fovea, Area temporalis, OKR, OMR, Zebrafish note: © 2022. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. abstract: Appropriate patterning of the retina during embryonic development is assumed to underlie the establishment of spatially localised specialisations that mediate the perception of specific visual features. For example, in zebrafish, an area involved in high acuity vision (HAA) is thought to be present in the ventro-temporal retina. Here, we show that the interplay of the transcription factor Rx3 with Fibroblast Growth Factor and Hedgehog signals initiates and restricts foxd1 expression to the prospective temporal retina, initiating naso-temporal regionalisation of the retina. Abrogation of Foxd1 results in the loss of temporal and expansion of nasal retinal character, and consequent absence of the HAA. These structural defects correlate with severe visual defects, as assessed in optokinetic and optomotor response assays. In contrast, optokinetic responses are unaffected in the opposite condition, in which nasal retinal character is lost at the expense of expanded temporal character. Our study indicates that the establishment of temporal retinal character during early retinal development is required for the specification of the HAA, and suggests a prominent role of the temporal retina in controlling specific visual functions. date: 2022-12-15 date_type: published publisher: The Company of Biologists official_url: https://doi.org/10.1242/dev.200938 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1997217 doi: 10.1242/dev.200938 medium: Print-Electronic pii: 285946 lyricists_name: Wilson, Stephen lyricists_name: Gestri, Gaia lyricists_name: Bianco, Isaac lyricists_id: SWILS65 lyricists_id: GGEST91 lyricists_id: IHBIA26 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner funding_acknowledgements: 213928/Z/18/Z [Wellcome Trust]; MR/L003775/1 [Medical Research Council] full_text_status: public publication: Development volume: 149 number: 24 article_number: dev200938 event_location: England citation: Hernández-Bejarano, M; Gestri, G; Monfries, C; Tucker, L; Dragomir, EI; Bianco, IH; Bovolenta, P; ... Cavodeassi, F; + view all <#> Hernández-Bejarano, M; Gestri, G; Monfries, C; Tucker, L; Dragomir, EI; Bianco, IH; Bovolenta, P; Wilson, SW; Cavodeassi, F; - view fewer <#> (2022) Foxd1-dependent induction of a temporal retinal character is required for visual function. Development , 149 (24) , Article dev200938. 10.1242/dev.200938 <https://doi.org/10.1242/dev.200938>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10162404/1/DownloadCombinedArticleAndSupplmentPdf.pdf