eprintid: 10161354 rev_number: 7 eprint_status: archive userid: 699 dir: disk0/10/16/13/54 datestamp: 2022-12-06 11:30:04 lastmod: 2024-11-11 16:38:05 status_changed: 2022-12-06 11:30:04 type: article metadata_visibility: show sword_depositor: 699 creators_name: Garcia-Segura, Monica Emili creators_name: Perez-Rodriguez, Diego creators_name: Chambers, Darren creators_name: Jaunmuktane, Zane creators_name: Proukakis, Christos title: Somatic SNCA Copy Number Variants in Multiple System Atrophy Are Related to Pathology and Inclusions ispublished: inpress subjects: UCH divisions: UCL divisions: B02 divisions: C07 divisions: D07 divisions: F84 keywords: alpha-synuclein, mosaicism, multiple system atrophy, snca, somatic mutation note: © 2022 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. abstract: BACKGROUND: Somatic α-synuclein (SNCA) copy number variants (CNVs, specifically gains) occur in multiple system atrophy (MSA) and Parkinson's disease brains. OBJECTIVE: The aim was to compare somatic SNCA CNVs in MSA subtypes (striatonigral degeneration [SND] and olivopontocerebellar atrophy [OPCA]) and correlate with inclusions. METHODS: We combined fluorescent in situ hybridization with immunofluorescence for α-synuclein and in some cases oligodendrocyte marker tubulin polymerization promoting protein (TPPP). RESULTS: We analyzed one to three brain regions from 24 MSA cases (13 SND, 11 OPCA). In a region preferentially affected in one subtype (putamen in SND, cerebellum in OPCA), mosaicism was higher in that subtype, and cells with CNVs were 4.2 times more likely to have inclusions. In the substantia nigra, nonpigmented cells with CNVs and TPPP were about six times more likely to have inclusions. CONCLUSIONS: The correlation between SNCA CNVs and pathology (at a regional level) and inclusions (at a single-cell level) suggests a role for somatic SNCA CNVs in MSA pathogenesis. © 2022 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. date: 2022-11-30 date_type: published publisher: Wiley official_url: https://doi.org/10.1002/mds.29291 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1993399 doi: 10.1002/mds.29291 medium: Print-Electronic lyricists_name: Proukakis, Christos lyricists_name: Jaunmuktane, Zane lyricists_id: CPROU78 lyricists_id: ZJAUN70 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner funding_acknowledgements: [Multiple System Atrophy Trust] full_text_status: public publication: Movement Disorders event_location: United States issn: 0885-3185 citation: Garcia-Segura, Monica Emili; Perez-Rodriguez, Diego; Chambers, Darren; Jaunmuktane, Zane; Proukakis, Christos; (2022) Somatic SNCA Copy Number Variants in Multiple System Atrophy Are Related to Pathology and Inclusions. Movement Disorders 10.1002/mds.29291 <https://doi.org/10.1002/mds.29291>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10161354/1/Somatic%20SNCA%20Copy%20Number%20Variants%20in%20Multiple%20System%20Atrophy.pdf